2011 |
Garcia-Beltran, O; Mena, N; Perez, E G; Cassels, B K; Nunez, M; Werlinger, F; Zavala, D; Aliaga, M E; Pavez, P The Development of a Fluorescence Turn-on Sensor for Cysteine, Glutathione and Other Biothiols. A Kinetic Study Artículo de revista Tetrahedron Letters, 52 (49), pp. 6606-6609, 2011, ISSN: 0040-4039. Resumen | Enlaces | BibTeX | Etiquetas: addition, biothiols, coumarin-based derivatives, disease, fluorescence homocysteine, michael probe, probes, thiols, water @article{RN17e, title = {The Development of a Fluorescence Turn-on Sensor for Cysteine, Glutathione and Other Biothiols. A Kinetic Study}, author = { O. Garcia-Beltran and N. Mena and E.G. Perez and B.K. Cassels and M. Nunez and F. Werlinger and D. Zavala and M.E. Aliaga and P. Pavez}, url = {/brokenurl#<Go to ISI>://WOS:000297442400022}, doi = {10.1016/j.tetlet.2011.09.137}, issn = {0040-4039}, year = {2011}, date = {2011-01-01}, journal = {Tetrahedron Letters}, volume = {52}, number = {49}, pages = {6606-6609}, publisher = {2011 Elsevier Ltd.}, abstract = {Two fluorescence probes for the detection of cysteine (Cys), glutathione (GSH) and other biothiols, such as homocysteine (Hcy) and cysteinyl-glycine (Cys-Gly), were developed. These molecular probes are coumarin-based derivatives containing a chalcone-like moiety that reacts with biothiols through a Michael addition reaction, leading to strong fluorescence enhancements. The reactivity of the tested biothiols toward both probes (ChC1 and ChC2) follows the order Cys > GSH > Hcy > Cys-Gly, ChC1 being less reactive than ChC2. Possible interference with other amino acids was assessed. ChC1 and ChC2 display a highly selective fluorescence enhancement with thiols, allowing these probes to be used for fluorimetric thiol determination in SH-SY5Y cells.}, keywords = {addition, biothiols, coumarin-based derivatives, disease, fluorescence homocysteine, michael probe, probes, thiols, water}, pubstate = {published}, tppubtype = {article} } Two fluorescence probes for the detection of cysteine (Cys), glutathione (GSH) and other biothiols, such as homocysteine (Hcy) and cysteinyl-glycine (Cys-Gly), were developed. These molecular probes are coumarin-based derivatives containing a chalcone-like moiety that reacts with biothiols through a Michael addition reaction, leading to strong fluorescence enhancements. The reactivity of the tested biothiols toward both probes (ChC1 and ChC2) follows the order Cys > GSH > Hcy > Cys-Gly, ChC1 being less reactive than ChC2. Possible interference with other amino acids was assessed. ChC1 and ChC2 display a highly selective fluorescence enhancement with thiols, allowing these probes to be used for fluorimetric thiol determination in SH-SY5Y cells. |
2011 |
The Development of a Fluorescence Turn-on Sensor for Cysteine, Glutathione and Other Biothiols. A Kinetic Study Artículo de revista Tetrahedron Letters, 52 (49), pp. 6606-6609, 2011, ISSN: 0040-4039. |