2015 |
Toral, M I; Nacaratte, F; Nova, F Determination of Etonogestrel and Ethinyl Estradiol from an Intrauterine Contraceptive Ring by Extraction and Derivative Spectrophotometry Artículo de revista Analytical Letters, 48 (6), pp. 1009-1020, 2015, ISSN: 0003-2719. Resumen | Enlaces | BibTeX | Etiquetas: contraceptive cyproterone-acetate, desogestrel, estradiol, ethinyl etonogestrel, formulation, intrauterine mass-spectrometry, pharmaceutical plasma properties, release ring, spectrophotometry @article{RN231, title = {Determination of Etonogestrel and Ethinyl Estradiol from an Intrauterine Contraceptive Ring by Extraction and Derivative Spectrophotometry}, author = { M.I. Toral and F. Nacaratte and F. Nova}, url = {/brokenurl#<Go to ISI>://WOS:000348519800011}, doi = {10.1080/00032719.2014.968930}, issn = {0003-2719}, year = {2015}, date = {2015-01-01}, journal = {Analytical Letters}, volume = {48}, number = {6}, pages = {1009-1020}, abstract = {A new method for the determination and extraction of ethinyl estradiol and etonogestrel was developed for a pharmaceutical product consisting of a solid copolymer intrauterine ring for subsequent simultaneous quantification by ultraviolet-visible derivative spectrophotometry. The spectral variables were optimized for first derivative spectrophotometry with a smoothing factor of 4000 and an amplification factor of 10,000. A wavelength of 291.5nm was selected for the determination of ethinyl estradiol by a graphical method, while for etonogestrel, zero-crossing was used at 249.5nm. The limits of detection and quantification for ethinyl estradiol were 9.5x10(-7) and 9.0x10(-6)mol/L and 1.1x10(-6) and 3.2x10(-6)mol/L for etonogestrel, respectively. For the extraction method, the variables affecting the analytical signal were the extraction solvent, temperature, and extraction time; optimized conditions were 180min at 76 +/- 2 degrees C in acetonitrile. The method was applied successfully for the first time to analyze intrauterine contraceptive rings. The sample contained 11.7mg etonogestrel and 2.7mg ethinyl estradiol; the recoveries were 93.6 +/- 1.1% etonogestrel and 97.9 +/- 1.5% ethinyl estradiol relative to the nominal concentration. The importance of this method involves its implementation in pharmaceutical laboratories.}, keywords = {contraceptive cyproterone-acetate, desogestrel, estradiol, ethinyl etonogestrel, formulation, intrauterine mass-spectrometry, pharmaceutical plasma properties, release ring, spectrophotometry}, pubstate = {published}, tppubtype = {article} } A new method for the determination and extraction of ethinyl estradiol and etonogestrel was developed for a pharmaceutical product consisting of a solid copolymer intrauterine ring for subsequent simultaneous quantification by ultraviolet-visible derivative spectrophotometry. The spectral variables were optimized for first derivative spectrophotometry with a smoothing factor of 4000 and an amplification factor of 10,000. A wavelength of 291.5nm was selected for the determination of ethinyl estradiol by a graphical method, while for etonogestrel, zero-crossing was used at 249.5nm. The limits of detection and quantification for ethinyl estradiol were 9.5x10(-7) and 9.0x10(-6)mol/L and 1.1x10(-6) and 3.2x10(-6)mol/L for etonogestrel, respectively. For the extraction method, the variables affecting the analytical signal were the extraction solvent, temperature, and extraction time; optimized conditions were 180min at 76 +/- 2 degrees C in acetonitrile. The method was applied successfully for the first time to analyze intrauterine contraceptive rings. The sample contained 11.7mg etonogestrel and 2.7mg ethinyl estradiol; the recoveries were 93.6 +/- 1.1% etonogestrel and 97.9 +/- 1.5% ethinyl estradiol relative to the nominal concentration. The importance of this method involves its implementation in pharmaceutical laboratories. |
2015 |
Determination of Etonogestrel and Ethinyl Estradiol from an Intrauterine Contraceptive Ring by Extraction and Derivative Spectrophotometry Artículo de revista Analytical Letters, 48 (6), pp. 1009-1020, 2015, ISSN: 0003-2719. |