Publicaciones 2011-2020

@article{RN410,
title = {Exploring the Influence of Diels-Alder Linker Length on Photothermal Molecule Release from Gold Nanorods},
author = { C. Vetterlein and R. Vasquez and K. Bolanos and G.A. Acosta and F. Guzman and F. Albericio and F. Celis and M. Campos-Vallette and M.J. Kogan and E. Araya},
url = {/brokenurl#<Go to ISI>://WOS:000432505900038},
doi = {10.1016/j.colsurfb.2018.03.021},
issn = {0927-7765},
year = {2018},
date = {2018-01-01},
journal = {Colloids and Surfaces B-Biointerfaces},
volume = {166},
pages = {323-329},
publisher = {2018 Elsevier B.V.},
abstract = {We studied the photothermal release of carboxyfluorescein (CF) linked to the gold surface of gold nanorods (GNRs) by two Diels-Alder adducts of different lengths (n = 4 and n = 9). The functionalized GNRs were irradiated with infrared light to produce photothermal release of CF by a retro-Diels-Alder reaction. The adducts were chemisorbed on the GNRs and the functionalized nanoparticles were characterized by UV-vis, DLS, zeta potential and Raman and surface-enhanced Raman spectroscopy (SEAS). On the basis of the degree of nanoparticle functionalization and the SERS results, we inferred the orientation of CF on the surface of the gold nanoparticle. Moreover, we determined the photothermal release profiles of CF from the gold surface by laser irradiation. The release was faster for the longer linker (n = 9). SERS revealed that, for the shorter linker (n = 4), molecules are oriented perpendicularly with respect to the gold surface, thereby maintaining the CF far from the surface. In contrast, the longer linker was observed to be tilted, thus maintaining CF close to the gold surface and therefore potentially favoring the photothermal transfer of energy. These results are relevant for the future development of the spatial and temporal controlled release of drugs by means of gold nanoparticles.},
keywords = {controlled drug-delivery, effect, enhanced fluorescence, micelles, nanoparticles, peptide photothermal polymeric raman-scattering, release, spatial temporal},
pubstate = {published},
tppubtype = {article}
}

@article{RN196,
title = {Molecular Characterization and in Silico Analysis of the Pheromone-Binding Protein of the European Grapevine Moth Lobesia Botrana (Denis & Schiffermuller) (Lepidoptera, Tortricidae)},
author = { A. Mutis and R. Palma and H. Venthur and P. Iturriaga-Vasquez and M. Faundez-Parraguez and R. Mella-Herrera and D. Kontodimas and C. Lobos and A. Quiroz},
url = {/brokenurl#<Go to ISI>://WOS:000335640500010},
doi = {10.1007/s13744-014-0212-2},
issn = {1519-566x},
year = {2014},
date = {2014-01-01},
journal = {Neotropical Entomology},
volume = {43},
number = {3},
pages = {266-275},
abstract = {The European grapevine moth Lobesia botrana (Denis & Schiffermuller) is an economically important insect in Europe. The species invaded vineyards in Chile, Argentina, and California during 2008-2010 causing severe problems. A major component of the sex pheromone, (E,Z)-7,9-dodecadienyl acetate (E7,Z9-12:Ac), is used in a mating disruption technique when grapevine moth populations are low or to monitor pest numbers. It is thought that these sexual pheromones are blends of volatiles that typically are specific to a species and are transported in the insect antenna by pheromone-binding proteins (PBPs) across the sensillar lymph to the olfactory receptors. Currently, an increasing number of Lepidopteran PBPs are being identified and cloned. However, there are no studies of the olfactory system and of proteins involved in the olfactory perception of L. botrana at the molecular level. In the present study, we report, for the first time, the sequence of a PBP from L. botrana (LbotPBP), which was determined using reverse transcription technology. Homology modeling was used to generate the three-dimensional protein structure. The model suggests that PBP consists of six alpha-helices as follows: Lys2-Met23 (alpha 1), Thr28-Phe36 (alpha 2), Arg46-Leu59 (alpha 3), His70-Asn80 (alpha 4), Glu84-Asn100 (alpha 5), and Cys108-Lys125 (alpha 6), held together by three disulfide bridges, Cys19-Cys54, Cys50-Cys108, and Cys97-Cys117. Docking simulations based on this model suggested that Trp114 is a keywords residue in the recognition of acetate pheromones, such as E7,Z9-12:Ac. In silico results in this study are consistent with previous findings in which E7,Z9-12:Ac acts as the most active compound in behavioral and electroantennographic assays.},
keywords = {alignment, anopheles-gambiae, antheraea-polyphemus, binding bombyx-mori, chemical crystal-structure, docking, ecology, ligand lymantria-dispar, modeling, molecular multiple nmr pheromone-binding protein, release, sequence sex-pheromone, site, structure, z)-7},
pubstate = {published},
tppubtype = {article}
}