2012 |
Carcamo, J J; Aliaga, A E; Clavijo, E; Branes, M; Campos-Vallette, M Raman Study of the Shockwave Effect on Collagens Artículo de revista Spectrochimica Acta Part a-Molecular and Biomolecular Spectroscopy, 86 , pp. 360-365, 2012, ISSN: 1386-1425. Resumen | Enlaces | BibTeX | Etiquetas: amino-acids, collagens, conformation, dependence, infrared-spectra, raman, scattering, shockwaves, spectroscopy, time tissues @article{carcamo2012raman, title = {Raman Study of the Shockwave Effect on Collagens}, author = { J.J. Carcamo and A.E. Aliaga and E. Clavijo and M. Branes and M. Campos-Vallette}, url = {/brokenurl#<Go to ISI>://WOS:000300515500052}, doi = {10.1016/j.saa.2011.10.049}, issn = {1386-1425}, year = {2012}, date = {2012-01-01}, journal = {Spectrochimica Acta Part a-Molecular and Biomolecular Spectroscopy}, volume = {86}, pages = {360-365}, publisher = {2011 Elsevier B.V.}, abstract = {The Raman spectra (1800-200 cm(-1)) of isolated dried collagen types land ill were recorded at different times after shockwave (SW) application in aqueous media. SWs were applied in a single session. One week after the SW application the vibrational data analysis indicates changes in the conformation of the collagens; orientational changes are also inferred. During the next three weeks collagens tended to recover the conformation and orientation existing before SW application.}, keywords = {amino-acids, collagens, conformation, dependence, infrared-spectra, raman, scattering, shockwaves, spectroscopy, time tissues}, pubstate = {published}, tppubtype = {article} } The Raman spectra (1800-200 cm(-1)) of isolated dried collagen types land ill were recorded at different times after shockwave (SW) application in aqueous media. SWs were applied in a single session. One week after the SW application the vibrational data analysis indicates changes in the conformation of the collagens; orientational changes are also inferred. During the next three weeks collagens tended to recover the conformation and orientation existing before SW application. |
Carcamo, J J; Aliaga, A E; Clavijo, E; Branes, M; Campos-Vallette, M Raman and Surface-Enhanced Raman Scattering in the Study of Human Rotator Cuff Tissues after Shock Wave Treatment Artículo de revista Journal of Raman Spectroscopy, 43 (2), pp. 248-254, 2012, ISSN: 0377-0486. Resumen | Enlaces | BibTeX | Etiquetas: amino-acids, collagen, collagens, cuff, gold infrared-spectra, nanoparticles, raman, rotator sers, shockwaves, silver, single-molecule, spectroscopy @article{RN39i, title = {Raman and Surface-Enhanced Raman Scattering in the Study of Human Rotator Cuff Tissues after Shock Wave Treatment}, author = { J.J. Carcamo and A.E. Aliaga and E. Clavijo and M. Branes and M. Campos-Vallette}, url = {/brokenurl#<Go to ISI>://WOS:000300836900011}, doi = {10.1002/jrs.3019}, issn = {0377-0486}, year = {2012}, date = {2012-01-01}, journal = {Journal of Raman Spectroscopy}, volume = {43}, number = {2}, pages = {248-254}, publisher = {2011 John Wiley & Sons, Ltd.}, abstract = {Important improvements of diseases of the rotator cuff supraspinatus tendons are seen after shock wave (SW) treatment. Neo-angiogenesis stimulation and hypercellularization result from short periods of treatment. The present work is an attempt to provide a first approach to these bioprocesses, most likely associated with structural aspects resulting from biochemical changes brought about by the SW. Immunohistochemical data indicate that collagen areas in the tissues are influenced the most by the SW. Presence of additional collagens I and III by the SW treatment is inferred from an observed increase of the tissue's tinctorial properties. The tools selected for our studies are Raman spectroscopy and the ultrasensitive surface-enhanced Raman scattering (SERS). Here we extract information from 1016 SERS spectra of 52 biopsies of human tendon tissues on Ag nanoparticles before and after the SW treatment. The spectral information is analyzed on the basis of Raman and SERS data of collagen types I and III and their most abundant amino acid components. SERS spectra of tissues reveal the presence of characteristic modes related mainly to amino acids. It has been found that the main differences between both tissue samples could be correlated with the structural conformational aspects of collagen.}, keywords = {amino-acids, collagen, collagens, cuff, gold infrared-spectra, nanoparticles, raman, rotator sers, shockwaves, silver, single-molecule, spectroscopy}, pubstate = {published}, tppubtype = {article} } Important improvements of diseases of the rotator cuff supraspinatus tendons are seen after shock wave (SW) treatment. Neo-angiogenesis stimulation and hypercellularization result from short periods of treatment. The present work is an attempt to provide a first approach to these bioprocesses, most likely associated with structural aspects resulting from biochemical changes brought about by the SW. Immunohistochemical data indicate that collagen areas in the tissues are influenced the most by the SW. Presence of additional collagens I and III by the SW treatment is inferred from an observed increase of the tissue's tinctorial properties. The tools selected for our studies are Raman spectroscopy and the ultrasensitive surface-enhanced Raman scattering (SERS). Here we extract information from 1016 SERS spectra of 52 biopsies of human tendon tissues on Ag nanoparticles before and after the SW treatment. The spectral information is analyzed on the basis of Raman and SERS data of collagen types I and III and their most abundant amino acid components. SERS spectra of tissues reveal the presence of characteristic modes related mainly to amino acids. It has been found that the main differences between both tissue samples could be correlated with the structural conformational aspects of collagen. |
2012 |
Raman Study of the Shockwave Effect on Collagens Artículo de revista Spectrochimica Acta Part a-Molecular and Biomolecular Spectroscopy, 86 , pp. 360-365, 2012, ISSN: 1386-1425. |
Raman and Surface-Enhanced Raman Scattering in the Study of Human Rotator Cuff Tissues after Shock Wave Treatment Artículo de revista Journal of Raman Spectroscopy, 43 (2), pp. 248-254, 2012, ISSN: 0377-0486. |