2015 |
Toral, M I; Sabay, T; Orellana, S L; Richter, P Determination of Oxytetracycline from Salmon Muscle and Skin by Derivative Spectrophotometry Artículo de revista Journal of Aoac International, 98 (3), pp. 559-565, 2015, ISSN: 1060-3271. Resumen | Enlaces | BibTeX | Etiquetas: antibiotics, aquaculture, detection, fluorescence liquid-chromatography, performance residues, samples tetracycline @article{RN232, title = {Determination of Oxytetracycline from Salmon Muscle and Skin by Derivative Spectrophotometry}, author = { M.I. Toral and T. Sabay and S.L. Orellana and P. Richter}, url = {/brokenurl#<Go to ISI>://WOS:000356903400004}, doi = {10.5740/jaoacint.14-027}, issn = {1060-3271}, year = {2015}, date = {2015-01-01}, journal = {Journal of Aoac International}, volume = {98}, number = {3}, pages = {559-565}, abstract = {A method was developed for the identification and quantification of oxytetracycline residues present in salmon muscle and skin using UV-Vis derivative spectrophotometry. With this method, it was possible to reduce the number of steps in the procedure typically required for instrumental analysis of a sample. The spectral variables, order of the derivative, scale factor, smoothing factor, and analytical wavelength were optimized using standard solutions of oxytetracycline dissolved in 900 mg/L oxalic acid in methanol. The matrix effect was significant; therefore, quantification for oxytetracycline residues was carried out using drug-free salmon muscle and skin samples fortified with oxytetracycline. The LOD and LOQ were found to be 271 and 903 mu g/kg, respectively. The precision and accuracy of the method were validated using drug-free salmon muscle and skin tissues fortified at three different concentrations (8, 16, and 32 mg/kg) on 3 different days. The recoveries at all fortified concentrations were between 90 and 105%, and RSDs in all cases were less than 6.5%. This method can be used to screen out compliant samples and thereby reduce the number of suspect positive samples that will require further confirmatory analysis.}, keywords = {antibiotics, aquaculture, detection, fluorescence liquid-chromatography, performance residues, samples tetracycline}, pubstate = {published}, tppubtype = {article} } A method was developed for the identification and quantification of oxytetracycline residues present in salmon muscle and skin using UV-Vis derivative spectrophotometry. With this method, it was possible to reduce the number of steps in the procedure typically required for instrumental analysis of a sample. The spectral variables, order of the derivative, scale factor, smoothing factor, and analytical wavelength were optimized using standard solutions of oxytetracycline dissolved in 900 mg/L oxalic acid in methanol. The matrix effect was significant; therefore, quantification for oxytetracycline residues was carried out using drug-free salmon muscle and skin samples fortified with oxytetracycline. The LOD and LOQ were found to be 271 and 903 mu g/kg, respectively. The precision and accuracy of the method were validated using drug-free salmon muscle and skin tissues fortified at three different concentrations (8, 16, and 32 mg/kg) on 3 different days. The recoveries at all fortified concentrations were between 90 and 105%, and RSDs in all cases were less than 6.5%. This method can be used to screen out compliant samples and thereby reduce the number of suspect positive samples that will require further confirmatory analysis. |
2015 |
Determination of Oxytetracycline from Salmon Muscle and Skin by Derivative Spectrophotometry Artículo de revista Journal of Aoac International, 98 (3), pp. 559-565, 2015, ISSN: 1060-3271. |