2017 |
Torres-Benitez, A; Rivera-Montalvo, M; Sepulveda, B; Castro, O N; Nagles, E; Simirgiotis, M J; Garcia-Beltran, O; Areche, C Metabolomic Analysis of Two Parmotrema Lichens: P-Robustum (Degel.) Hale and P-Andinum (Mull. Arg.) Hale Using Uhplc-Esi-Ot-Ms-Ms Artículo de revista Molecules, 22 (11), 2017, ISSN: 1420-3049. Resumen | Enlaces | BibTeX | Etiquetas: constituents, electrospray, lichens, metabolites, metabolomic, ms/ms, nyl. orbitrap, parmotrema, phenolic-compounds, secondary uhplc-ms-ms @article{RN334, title = {Metabolomic Analysis of Two Parmotrema Lichens: P-Robustum (Degel.) Hale and P-Andinum (Mull. Arg.) Hale Using Uhplc-Esi-Ot-Ms-Ms}, author = { A. Torres-Benitez and M. Rivera-Montalvo and B. Sepulveda and O.N. Castro and E. Nagles and M.J. Simirgiotis and O. Garcia-Beltran and C. Areche}, url = {/brokenurl#<Go to ISI>://WOS:000416528400063}, doi = {10.3390/molecules22111861}, issn = {1420-3049}, year = {2017}, date = {2017-01-01}, journal = {Molecules}, volume = {22}, number = {11}, abstract = {Lichens are symbiotic associations of fungi with microalgae and/or cyanobacteria. Lichens belonging to the Parmeliaceae family comprise 2700 species of lichens, including the Parmotrema genus which is composed of 300 species. The metabolites of this genus include depsides, depsidones, phenolics, polysaccharides, lipids, diphenylethers and dibenzofurans, which are responsible for the biological activities reported including antidiabetic, antihelmintic, anticancer, antioxidant, antibacterial, anti-inflammatory, antimitotic, antitumoral, antifungal, and antioxidant enzyme inhibitory. Due to scarce knowledge of metabolomic profiles of Parmotrema species (P. andinum and P. robustum), a full metabolome study based on ultra-high performance liquid chromatography- diode array detector-electrospray ionization-quadrupole-orbitrap-mass-spectrometry (UHPLC-DAD-ESI-Q-orbitrap MS) was performed for a comprehensive characterization of their substances. From the methanolic extracts of these species, a total of 54 metabolites were identified for the first time using this hyphenated technique, including thirty compounds in P. andinum, and thirty-seven in P. robustum. Moreover, two compounds were not identified as known compounds, and could be new structures, according to our data. This report shows that this technique is effective and accurate for rapid chemical identification of lichen substances and the compounds identified could serve as chemotaxonomic markers to differentiate these ruffle lichens.}, keywords = {constituents, electrospray, lichens, metabolites, metabolomic, ms/ms, nyl. orbitrap, parmotrema, phenolic-compounds, secondary uhplc-ms-ms}, pubstate = {published}, tppubtype = {article} } Lichens are symbiotic associations of fungi with microalgae and/or cyanobacteria. Lichens belonging to the Parmeliaceae family comprise 2700 species of lichens, including the Parmotrema genus which is composed of 300 species. The metabolites of this genus include depsides, depsidones, phenolics, polysaccharides, lipids, diphenylethers and dibenzofurans, which are responsible for the biological activities reported including antidiabetic, antihelmintic, anticancer, antioxidant, antibacterial, anti-inflammatory, antimitotic, antitumoral, antifungal, and antioxidant enzyme inhibitory. Due to scarce knowledge of metabolomic profiles of Parmotrema species (P. andinum and P. robustum), a full metabolome study based on ultra-high performance liquid chromatography- diode array detector-electrospray ionization-quadrupole-orbitrap-mass-spectrometry (UHPLC-DAD-ESI-Q-orbitrap MS) was performed for a comprehensive characterization of their substances. From the methanolic extracts of these species, a total of 54 metabolites were identified for the first time using this hyphenated technique, including thirty compounds in P. andinum, and thirty-seven in P. robustum. Moreover, two compounds were not identified as known compounds, and could be new structures, according to our data. This report shows that this technique is effective and accurate for rapid chemical identification of lichen substances and the compounds identified could serve as chemotaxonomic markers to differentiate these ruffle lichens. |
2016 |
Simirgiotis, M J; Quispe, C; Borquez, J; Areche, C; Sepulveda, B Molecules, 21 (1), 2016, ISSN: 1420-3049. Resumen | Enlaces | BibTeX | Etiquetas: activity, antioxidant capacity, chile, chilean de esi-ms, flavonoids, fruits, hplc-dad, ms/ms, northern orbitrap parts pascua, pda, pears, pera phenolics, proanthocyanidins, products, uhplc-ms @article{RN289, title = {Fast Detection of Phenolic Compounds in Extracts of Easter Pears (Pyrus Communis) from the Atacama Desert by Ultrahigh-Performance Liquid Chromatography and Mass Spectrometry (Uhplc-Q/Orbitrap/Ms/Ms)}, author = { M.J. Simirgiotis and C. Quispe and J. Borquez and C. Areche and B. Sepulveda}, url = {/brokenurl#<Go to ISI>://WOS:000369486800014}, doi = {10.3390/molecules21010092}, issn = {1420-3049}, year = {2016}, date = {2016-01-01}, journal = {Molecules}, volume = {21}, number = {1}, abstract = {A small Chilean variety of pears growing in the town of Toconao, an oasis located at the northeastern edge of the Salar de Atacama, northern Chile, was studied by means of modern PDA and high resolution mass spectral data (UHPLC-PDA-HESI-orbitrap-MS/MS). In addition, the antioxidant features of the fruits were compared with the varieties Packhman's Triumph and Abate Fetel and correlated with the presence of phenolic compounds. The non-pigmented phenolics were fingerprinted and related to the antioxidant capacities measured by the bleaching of the DPPH radical, the ferric reducing antioxidant power (FRAP), the superoxide anion scavenging activity assay (SA), and total content of phenolics and flavonoids measured by spectroscopic methods. The machine allowed a fast separation of 15 min employing a flow rate of 1 mL per minute and could accurately identify 25 compounds, including several isorhamnetin derivatives and phenolic acids, present in the peel and pulps of this Chilean variety for the first time. The compounds were monitored using a wavelength range of 210-800 nm. The native small Chilean pear showed the highest antioxidant activity measured as the bleaching of the DPPH radical, the ferric reducing antioxidant power and superoxide anion scavenging activity (8.61 +/- 0.65 g/mL, 712.63 +/- 12.12 micromols trolox equivalents (mol/TE)/100 g FW, and 82.89% +/- 2.52% at 100 g/mL, respectively).}, keywords = {activity, antioxidant capacity, chile, chilean de esi-ms, flavonoids, fruits, hplc-dad, ms/ms, northern orbitrap parts pascua, pda, pears, pera phenolics, proanthocyanidins, products, uhplc-ms}, pubstate = {published}, tppubtype = {article} } A small Chilean variety of pears growing in the town of Toconao, an oasis located at the northeastern edge of the Salar de Atacama, northern Chile, was studied by means of modern PDA and high resolution mass spectral data (UHPLC-PDA-HESI-orbitrap-MS/MS). In addition, the antioxidant features of the fruits were compared with the varieties Packhman's Triumph and Abate Fetel and correlated with the presence of phenolic compounds. The non-pigmented phenolics were fingerprinted and related to the antioxidant capacities measured by the bleaching of the DPPH radical, the ferric reducing antioxidant power (FRAP), the superoxide anion scavenging activity assay (SA), and total content of phenolics and flavonoids measured by spectroscopic methods. The machine allowed a fast separation of 15 min employing a flow rate of 1 mL per minute and could accurately identify 25 compounds, including several isorhamnetin derivatives and phenolic acids, present in the peel and pulps of this Chilean variety for the first time. The compounds were monitored using a wavelength range of 210-800 nm. The native small Chilean pear showed the highest antioxidant activity measured as the bleaching of the DPPH radical, the ferric reducing antioxidant power and superoxide anion scavenging activity (8.61 +/- 0.65 g/mL, 712.63 +/- 12.12 micromols trolox equivalents (mol/TE)/100 g FW, and 82.89% +/- 2.52% at 100 g/mL, respectively). |
2017 |
Metabolomic Analysis of Two Parmotrema Lichens: P-Robustum (Degel.) Hale and P-Andinum (Mull. Arg.) Hale Using Uhplc-Esi-Ot-Ms-Ms Artículo de revista Molecules, 22 (11), 2017, ISSN: 1420-3049. |
2016 |
Molecules, 21 (1), 2016, ISSN: 1420-3049. |