Publicaciones 2011-2020

@article{RN318,
title = {Fluorescence and Surface-Enhanced Vibrational Spectroscopies of Lawsone and Plumbagin},
author = { A.E. Aliaga and P. Leyton and E. Clavijo and M. Campos-Vallette},
url = {/brokenurl#<Go to ISI>://WOS:000405754400002},
doi = {10.1080/00387010.2016.1146772},
issn = {0038-7010},
year = {2016},
date = {2016-01-01},
journal = {Spectroscopy Letters},
volume = {49},
number = {5},
pages = {326-335},
abstract = {The natural dyes lawsone and plumbagin (1,4-naphthoquinones) were studied by using fluorescence, Raman, infrared (IR), surface-enhanced Raman scattering (SERS), and surface-enhanced IR absorption. From the absorption spectrum, it was possible to infer that the enol-lawsone tautomer concentration decreases in silver colloidal solution. Plumbagin dimers, both in water and in silver colloidal solution, were identified from the fluorescence band profile and surface-SERS spectrum. The SERS spectrum of lawsone was obtained by using silver colloidal solution after 12-hour settle; the keto and enol lawsone silver colloid interaction occurs. The assignment of Raman and IR bands of both dyes was calculated with density functional theory calculations. Only a monomeric structure of lawsone interacting with a silver cluster model was predicted. The monomer adopts a nearly coplanar orientation onto the silver surface; the shortest distance is 3.2 angstrom, suggesting an electrostatic interaction.},
keywords = {behavior, calculations, enhanced fluorescence, infrared-absorption, lawsone, nanoparticles, plumbagin, quinones raman raman-scattering, reduction, scattering, selection, sers, silver spectrum, surface theoretical},
pubstate = {published},
tppubtype = {article}
}

@article{RN132,
title = {Increase in the Astaxanthin Synthase Gene (Crts) Dose by in Vivo DNA Fragment Assembly in Xanthophyllomyces Dendrorhous},
author = { G. Contreras and S. Barahona and M.C. Rojas and M. Baeza and V. Cifuentes and J. Alcaino},
url = {/brokenurl#<Go to ISI>://WOS:000327426600001},
doi = {Unsp 84, 10.1186/1472-6750-13-84},
issn = {1472-6750},
year = {2013},
date = {2013-01-01},
journal = {Bmc Biotechnology},
volume = {13},
abstract = {Background: Xanthophyllomyces dendrorhous is a basidiomycetous yeast that is relevant to biotechnology, as it can synthesize the carotenoid astaxanthin. However, the astaxanthin levels produced by wild-type strains are low. Although different approaches for promoting increased astaxanthin production have been attempted, no commercially competitive results have been obtained thus far. A promising alternative to facilitate the production of carotenoids in this yeast involves the use of genetic modification. However, a major limitation is the few available molecular tools to manipulate X. dendrorhous., Results: In this work, the DNA assembler methodology that was previously described in Saccharomyces cerevisiae was successfully applied to assemble DNA fragments in vivo and integrate these fragments into the genome of X. dendrorhous by homologous recombination in only one transformation event. Using this method, the gene encoding astaxanthin synthase (crtS) was overexpressed in X. dendrorhous and a higher level of astaxanthin was produced., Conclusions: This methodology could be used to easily and rapidly overexpress individual genes or combinations of genes simultaneously in X. dendrorhous, eliminating numerous steps involved in conventional cloning methods.},
keywords = {astaxanthin beta-carotene, biosynthetic-pathway, carotenoid cloning, dendrorhous, DNA assembler, expression, growth mutants, overproducing oxygen, phaffia-rhodozyma, selection, strains, synthase, xanthophyllomyces},
pubstate = {published},
tppubtype = {article}
}