Publicaciones 2011-2020

@article{RN340,
title = {The Biosynthetic Gene Cluster for Andrastin a in Penicillium Roqueforti},
author = { J.F. Rojas-Aedo and C. Gil-Duran and A. Del-Cid and N. Valdes and P. Alamos and I. Vaca and R.O. Garcia-Rico and G. Levican and M. Tello and R. Chavez},
url = {/brokenurl#<Go to ISI>://WOS:000400641200002},
doi = {10.3389/fmicb.2017.00813},
issn = {1664-302x},
year = {2017},
date = {2017-01-01},
journal = {Frontiers in Microbiology},
volume = {8},
abstract = {Penicillium roqueforti is a filamentous fungus involved in the ripening of several kinds of blue cheeses. In addition, this fungus produces several secondary metabolites, including the meroterpenoid compound andrastin A, a promising antitumoral compound. However, to date the genomic cluster responsible for the biosynthesis of this compound in P. roqueforti has not been described. In this work, we have sequenced and annotated a genomic region of approximately 29.4 kbp (named the adr gene cluster) that is involved in the biosynthesis of andrastin A in P. roqueforti. This region contains ten genes, named adrA, adrC, adrD, adrE, adrF, adrG, adrH, adrI, adrJ and adrK. Interestingly, the adrB gene previously found in the adr cluster from P. chrysogenum, was found as a residual pseudogene in the adr cluster from P. roqueforti. RNA-mediated gene silencing of each of the ten genes resulted in significant reductions in andrastin A production, confirming that all of them are involved in the biosynthesis of this compound. Of particular interest was the adrC gene, encoding for a major facilitator superfamily transporter. According to our results, this gene is required for the production of andrastin A but does not have any role in its secretion to the extracellular medium. The identification of the adr cluster in P. roqueforti will be important to understand the molecular basis of the production of andrastin A, and for the obtainment of strains of P. roqueforti overproducing andrastin A that might be of interest for the cheese industry.},
keywords = {andrastin cells chrysogenum, cluster, fo-3929, fungal gene metabolism, metabolites, penicillium rna-mediated roqueforti, secondary secretion, silencing, strains, transporter},
pubstate = {published},
tppubtype = {article}
}

@article{RN290,
title = {Identification and Functional Analysis of the Mycophenolic Acid Gene Cluster of Penicillium Roqueforti},
author = { A. Del-Cid and C. Gil-Duran and I. Vaca and J.F. Rojas-Aedo and R.O. Garcia-Rico and G. Levican and R. Chavez},
url = {/brokenurl#<Go to ISI>://WOS:000367888100190},
doi = {10.1371/journal.pone.0147047},
issn = {1932-6203},
year = {2016},
date = {2016-01-01},
journal = {Plos One},
volume = {11},
number = {1},
abstract = {The filamentous fungus Penicillium roqueforti is widely known as the ripening agent of blue-veined cheeses. Additionally, this fungus is able to produce several secondary metabolites, including the meroterpenoid compound mycophenolic acid (MPA). Cheeses ripened with P. roqueforti are usually contaminated with MPA. On the other hand, MPA is a commercially valuable immunosuppressant. However, to date the molecular basis of the production of MPA by P. roqueforti is still unknown. Using a bioinformatic approach, we have identified a genomic region of approximately 24.4 kbp containing a seven-gene cluster that may be involved in the MPA biosynthesis in P. roqueforti. Gene silencing of each of these seven genes (named mpaA, mpaB, mpaC, mpaDE, mpaF, mpaG and mpaH) resulted in dramatic reductions in MPA production, confirming that all of these genes are involved in the biosynthesis of the compound. Interestingly, the mpaF gene, originally described in P. brevicompactum as a MPA self-resistance gene, also exerts the same function in P. roqueforti, suggesting that this gene has a dual function in MPA metabolism. The knowledge of the biosynthetic pathway of MPA in P. roqueforti will be important for the future control of MPA contamination in cheeses and the improvement of MPA production for commercial purposes.},
keywords = {biosynthesis, cheese, chrysogenum, dehydrogenase, expression, fungi, immunosuppressant, imp liquid-chromatography, mycotoxins, strains},
pubstate = {published},
tppubtype = {article}
}

@article{RN132,
title = {Increase in the Astaxanthin Synthase Gene (Crts) Dose by in Vivo DNA Fragment Assembly in Xanthophyllomyces Dendrorhous},
author = { G. Contreras and S. Barahona and M.C. Rojas and M. Baeza and V. Cifuentes and J. Alcaino},
url = {/brokenurl#<Go to ISI>://WOS:000327426600001},
doi = {Unsp 84, 10.1186/1472-6750-13-84},
issn = {1472-6750},
year = {2013},
date = {2013-01-01},
journal = {Bmc Biotechnology},
volume = {13},
abstract = {Background: Xanthophyllomyces dendrorhous is a basidiomycetous yeast that is relevant to biotechnology, as it can synthesize the carotenoid astaxanthin. However, the astaxanthin levels produced by wild-type strains are low. Although different approaches for promoting increased astaxanthin production have been attempted, no commercially competitive results have been obtained thus far. A promising alternative to facilitate the production of carotenoids in this yeast involves the use of genetic modification. However, a major limitation is the few available molecular tools to manipulate X. dendrorhous., Results: In this work, the DNA assembler methodology that was previously described in Saccharomyces cerevisiae was successfully applied to assemble DNA fragments in vivo and integrate these fragments into the genome of X. dendrorhous by homologous recombination in only one transformation event. Using this method, the gene encoding astaxanthin synthase (crtS) was overexpressed in X. dendrorhous and a higher level of astaxanthin was produced., Conclusions: This methodology could be used to easily and rapidly overexpress individual genes or combinations of genes simultaneously in X. dendrorhous, eliminating numerous steps involved in conventional cloning methods.},
keywords = {astaxanthin beta-carotene, biosynthetic-pathway, carotenoid cloning, dendrorhous, DNA assembler, expression, growth mutants, overproducing oxygen, phaffia-rhodozyma, selection, strains, synthase, xanthophyllomyces},
pubstate = {published},
tppubtype = {article}
}