Publicaciones 2011-2020

@article{RN347,
title = {Elucidation of Gibberellin Biosynthesis in Bacteria Reveals Convergent Evolution},
author = { R.S. Nett and M. Montanares and A. Marcassa and X. Lul and R. Nagel and T.C. Charles and P. Hedden and M.C. Rojas and R.J. Peters},
url = {/brokenurl#<Go to ISI>://WOS:000393267200015},
doi = {10.1038/Nchembio.2232},
issn = {1552-4450},
year = {2017},
date = {2017-01-01},
journal = {Nature Chemical Biology},
volume = {13},
number = {1},
pages = {69-74},
abstract = {Gibberellins (GAs) are crucial phytohormones involved in many aspects of plant growth and development, including plant-microbe interactions, which has led to GA production by plant-associated fungi and bacteria as well. While the GA biosynthetic pathways in plants and fungi have been elucidated and found to have arisen independently through convergent evolution, little has been uncovered about GA biosynthesis in bacteria. Some nitrogen-fixing, symbiotic, legume-associated rhizobia, including Bradyrhizobium japonicum-the symbiont of soybean and Sinorhizobium fredii-a broad-host-nodulating species-contain a putative GA biosynthetic operon, or gene cluster. Through functional characterization of five unknown genes, we demonstrate that this operon encodes the enzymes necessary to produce GA(9), thereby elucidating bacterial GA biosynthesis. The distinct nature of these enzymes indicates that bacteria have independently evolved a third biosynthetic pathway for GA production. Furthermore, our results also reveal a central biochemical logic that is followed in all three convergently evolved GA biosynthetic pathways.},
keywords = {artemisinin biosynthesis, bradyrhizobium-japonicum, cloning, cytochrome-p450 ent-kaurene, fungi, gene-cluster, green-revolution, identification, plants, rearrangement},
pubstate = {published},
tppubtype = {article}
}

@article{RN290,
title = {Identification and Functional Analysis of the Mycophenolic Acid Gene Cluster of Penicillium Roqueforti},
author = { A. Del-Cid and C. Gil-Duran and I. Vaca and J.F. Rojas-Aedo and R.O. Garcia-Rico and G. Levican and R. Chavez},
url = {/brokenurl#<Go to ISI>://WOS:000367888100190},
doi = {10.1371/journal.pone.0147047},
issn = {1932-6203},
year = {2016},
date = {2016-01-01},
journal = {Plos One},
volume = {11},
number = {1},
abstract = {The filamentous fungus Penicillium roqueforti is widely known as the ripening agent of blue-veined cheeses. Additionally, this fungus is able to produce several secondary metabolites, including the meroterpenoid compound mycophenolic acid (MPA). Cheeses ripened with P. roqueforti are usually contaminated with MPA. On the other hand, MPA is a commercially valuable immunosuppressant. However, to date the molecular basis of the production of MPA by P. roqueforti is still unknown. Using a bioinformatic approach, we have identified a genomic region of approximately 24.4 kbp containing a seven-gene cluster that may be involved in the MPA biosynthesis in P. roqueforti. Gene silencing of each of these seven genes (named mpaA, mpaB, mpaC, mpaDE, mpaF, mpaG and mpaH) resulted in dramatic reductions in MPA production, confirming that all of these genes are involved in the biosynthesis of the compound. Interestingly, the mpaF gene, originally described in P. brevicompactum as a MPA self-resistance gene, also exerts the same function in P. roqueforti, suggesting that this gene has a dual function in MPA metabolism. The knowledge of the biosynthetic pathway of MPA in P. roqueforti will be important for the future control of MPA contamination in cheeses and the improvement of MPA production for commercial purposes.},
keywords = {biosynthesis, cheese, chrysogenum, dehydrogenase, expression, fungi, immunosuppressant, imp liquid-chromatography, mycotoxins, strains},
pubstate = {published},
tppubtype = {article}
}

@article{RN180,
title = {Diversity of Cultivable Fungi Associated with Antarctic Marine Sponges and Screening for Their Antimicrobial, Antitumoral and Antioxidant Potential},
author = { M. Henriquez and K. Vergara and J. Norambuena and A. Beiza and F. Maza and P. Ubilla and I. Araya and R. Chavez and A. San-Martin and J. Darias and M.J. Darias and I. Vaca},
url = {/brokenurl#<Go to ISI>://WOS:000329248200007},
doi = {10.1007/s11274-013-1418-x},
issn = {0959-3993},
year = {2014},
date = {2014-01-01},
journal = {World Journal of Microbiology & Biotechnology},
volume = {30},
number = {1},
pages = {65-76},
abstract = {The diversity of sponge-associated fungi has been poorly investigated in remote geographical areas like Antarctica. In this study, 101 phenotypically different fungal isolates were obtained from 11 sponge samples collected in King George Island, Antarctica. The analysis of ITS sequences revealed that they belong to the phylum Ascomycota. Sixty-five isolates belong to the genera Geomyces, Penicillium, Epicoccum, Pseudeurotium, Thelebolus, Cladosporium, Aspergillus, Aureobasidium, Phoma, and Trichocladium but 36 isolates could not be identified at genus level. In order to estimate the potential of these isolates as producers of interesting bioactivities, antimicrobial, antitumoral and antioxidant activities of fungal culture extracts were assayed. Around 51 % of the extracts, mainly from the genus Geomyces and non identified relatives, showed antimicrobial activity against some of the bacteria tested. On the other hand, around 42 % of the extracts showed potent antitumoral activity, Geomyces sp. having the best performance. Finally, the potential of the isolated fungi as producers of antioxidant activity seems to be moderate. Our results suggest that fungi associated with Antarctic sponges, particularly Geomyces, would be valuable sources of antimicrobial and antitumoral compounds. To our knowledge, this is the first report describing the biodiversity and the metabolic potential of fungi associated with Antarctic marine sponges.},
keywords = {antarctic antimicrobial, antioxidant, antitumoral, bioactive coastal diversity, fungi, geomyces haliclona-simulans, identification, metabolites, microorganisms natural-products, penicillium, phylogenetic sp., sponges, suberites-zeteki, syndrome, waters, white-nose},
pubstate = {published},
tppubtype = {article}
}

@article{RN112,
title = {Biological Activity of Macromycetes Isolated from Chilean Subantarctic Ecosystems},
author = { R. Reinoso and D. Cajas-Madriaga and M. Martinez and A. San Martin and C. Perez and V. Fajardo and J. Becerra},
url = {/brokenurl#<Go to ISI>://WOS:000331238800020},
doi = {10.4067/S0717-97072013000400024},
issn = {0717-9707},
year = {2013},
date = {2013-01-01},
journal = {Journal of the Chilean Chemical Society},
volume = {58},
number = {4},
pages = {2016-2019},
abstract = {Twenty six compounds were identified by GC-MS analysis from culture broth of six Macromycetes growing in subantarctic forests in southern Chile: Mycena hialinotricha, Collybia butyracea, Inocybe geophylla, Entoloma nubigenum, Stropharia semiglobata and Psathyrella sp. Antifungal and antibacterial activity were evaluated through agar diffusion test. This assay showed bioactivity against Penicillium notatum, Fusarium oxysporum, Rhyzoctonia solani, Ceratocistys pilifera, Bacillus subtilis, Pseudomonas aeruginosa, Escherichia coli, Escherichia faecalis and Staphilococcus aureus.},
keywords = {activity, alkaloids and antibacterial, antifungal ecosystems, fungi, metabolites, secondary subantarctic},
pubstate = {published},
tppubtype = {article}
}