2017 |
Rojas-Aedo, J F; Gil-Duran, C; Del-Cid, A; Valdes, N; Alamos, P; Vaca, I; Garcia-Rico, R O; Levican, G; Tello, M; Chavez, R The Biosynthetic Gene Cluster for Andrastin a in Penicillium Roqueforti Artículo de revista Frontiers in Microbiology, 8 , 2017, ISSN: 1664-302x. Resumen | Enlaces | BibTeX | Etiquetas: andrastin cells chrysogenum, cluster, fo-3929, fungal gene metabolism, metabolites, penicillium rna-mediated roqueforti, secondary secretion, silencing, strains, transporter @article{RN340, title = {The Biosynthetic Gene Cluster for Andrastin a in Penicillium Roqueforti}, author = { J.F. Rojas-Aedo and C. Gil-Duran and A. Del-Cid and N. Valdes and P. Alamos and I. Vaca and R.O. Garcia-Rico and G. Levican and M. Tello and R. Chavez}, url = {/brokenurl#<Go to ISI>://WOS:000400641200002}, doi = {10.3389/fmicb.2017.00813}, issn = {1664-302x}, year = {2017}, date = {2017-01-01}, journal = {Frontiers in Microbiology}, volume = {8}, abstract = {Penicillium roqueforti is a filamentous fungus involved in the ripening of several kinds of blue cheeses. In addition, this fungus produces several secondary metabolites, including the meroterpenoid compound andrastin A, a promising antitumoral compound. However, to date the genomic cluster responsible for the biosynthesis of this compound in P. roqueforti has not been described. In this work, we have sequenced and annotated a genomic region of approximately 29.4 kbp (named the adr gene cluster) that is involved in the biosynthesis of andrastin A in P. roqueforti. This region contains ten genes, named adrA, adrC, adrD, adrE, adrF, adrG, adrH, adrI, adrJ and adrK. Interestingly, the adrB gene previously found in the adr cluster from P. chrysogenum, was found as a residual pseudogene in the adr cluster from P. roqueforti. RNA-mediated gene silencing of each of the ten genes resulted in significant reductions in andrastin A production, confirming that all of them are involved in the biosynthesis of this compound. Of particular interest was the adrC gene, encoding for a major facilitator superfamily transporter. According to our results, this gene is required for the production of andrastin A but does not have any role in its secretion to the extracellular medium. The identification of the adr cluster in P. roqueforti will be important to understand the molecular basis of the production of andrastin A, and for the obtainment of strains of P. roqueforti overproducing andrastin A that might be of interest for the cheese industry.}, keywords = {andrastin cells chrysogenum, cluster, fo-3929, fungal gene metabolism, metabolites, penicillium rna-mediated roqueforti, secondary secretion, silencing, strains, transporter}, pubstate = {published}, tppubtype = {article} } Penicillium roqueforti is a filamentous fungus involved in the ripening of several kinds of blue cheeses. In addition, this fungus produces several secondary metabolites, including the meroterpenoid compound andrastin A, a promising antitumoral compound. However, to date the genomic cluster responsible for the biosynthesis of this compound in P. roqueforti has not been described. In this work, we have sequenced and annotated a genomic region of approximately 29.4 kbp (named the adr gene cluster) that is involved in the biosynthesis of andrastin A in P. roqueforti. This region contains ten genes, named adrA, adrC, adrD, adrE, adrF, adrG, adrH, adrI, adrJ and adrK. Interestingly, the adrB gene previously found in the adr cluster from P. chrysogenum, was found as a residual pseudogene in the adr cluster from P. roqueforti. RNA-mediated gene silencing of each of the ten genes resulted in significant reductions in andrastin A production, confirming that all of them are involved in the biosynthesis of this compound. Of particular interest was the adrC gene, encoding for a major facilitator superfamily transporter. According to our results, this gene is required for the production of andrastin A but does not have any role in its secretion to the extracellular medium. The identification of the adr cluster in P. roqueforti will be important to understand the molecular basis of the production of andrastin A, and for the obtainment of strains of P. roqueforti overproducing andrastin A that might be of interest for the cheese industry. |
2016 |
Munoz-Perez, J; Leyton, P; Paipa, C; Soto, J P; Brunet, J; Gomez-Jeria, J S; Campos-Vallette, M Raman and Surface Enhanced Raman Scattering Study of the Orientation of Cruciform 9,10-Anthracene Thiophene and Furan Derivatives Deposited on a Gold Colloidal Surface Artículo de revista Journal of Molecular Structure, 1122 , pp. 198-204, 2016, ISSN: 0022-2860. Resumen | Enlaces | BibTeX | Etiquetas: cluster, cruciform, electronics, gold metal molecular monolayers, nanoparticles, pi-systems, polycyclic raman scattering, self-assembled sers, silver, spectroscopy, spectrum, surface-enhanced vibrational-spectra @article{RN331, title = {Raman and Surface Enhanced Raman Scattering Study of the Orientation of Cruciform 9,10-Anthracene Thiophene and Furan Derivatives Deposited on a Gold Colloidal Surface}, author = { J. Munoz-Perez and P. Leyton and C. Paipa and J.P. Soto and J. Brunet and J.S. Gomez-Jeria and M. Campos-Vallette}, url = {/brokenurl#<Go to ISI>://WOS:000381167800023}, doi = {10.1016/j.molstruc.2016.06.002}, issn = {0022-2860}, year = {2016}, date = {2016-01-01}, journal = {Journal of Molecular Structure}, volume = {1122}, pages = {198-204}, publisher = {2016 Elsevier B.V.}, abstract = {The 9,10-di(thiophen-2-yl)anthracene (TAT), 9,10-di(furan-2-yl)anthracene (FAF) and 2-[(10-(thiophen-2-yl)anthracen-9-yl)]furan (TAF) cruciform molecular systems were synthesized using one-step coupling reactions and structurally characterized via Raman, infrared, H-1 NMR, C-13 NMR and mass spectroscopies. The orientation of the analytes on a gold colloidal surface was inferred from a surface-enhanced Raman scattering (SERS) study. The metal surface interaction was driven by the S and O atoms of the thiophene and furan alpha-substituents, and the plane of the anthracene fragment remained parallel to the surface. Theoretical calculations based on a simplified molecular model for the analyte-surface interaction provide a good representation of the experimental data.}, keywords = {cluster, cruciform, electronics, gold metal molecular monolayers, nanoparticles, pi-systems, polycyclic raman scattering, self-assembled sers, silver, spectroscopy, spectrum, surface-enhanced vibrational-spectra}, pubstate = {published}, tppubtype = {article} } The 9,10-di(thiophen-2-yl)anthracene (TAT), 9,10-di(furan-2-yl)anthracene (FAF) and 2-[(10-(thiophen-2-yl)anthracen-9-yl)]furan (TAF) cruciform molecular systems were synthesized using one-step coupling reactions and structurally characterized via Raman, infrared, H-1 NMR, C-13 NMR and mass spectroscopies. The orientation of the analytes on a gold colloidal surface was inferred from a surface-enhanced Raman scattering (SERS) study. The metal surface interaction was driven by the S and O atoms of the thiophene and furan alpha-substituents, and the plane of the anthracene fragment remained parallel to the surface. Theoretical calculations based on a simplified molecular model for the analyte-surface interaction provide a good representation of the experimental data. |
2015 |
Gil-Duran, C; Rojas-Aedo, J F; Medina, E; Vaca, I; Garcia-Rico, R O; Villagran, S; Levican, G; Chavez, R The Pcz1 Gene, Which Encodes a Zn(Ii)(2)Cys(6) Protein, Is Involved in the Control of Growth, Conidiation, and Conidial Germination in the Filamentous Fungus Penicillium Roqueforti Artículo de revista Plos One, 10 (3), 2015, ISSN: 1932-6203. Resumen | Enlaces | BibTeX | Etiquetas: alpha-subunit, aspergillus-nidulans, chrysogenum, cluster, pga1 @article{RN240, title = {The Pcz1 Gene, Which Encodes a Zn(Ii)(2)Cys(6) Protein, Is Involved in the Control of Growth, Conidiation, and Conidial Germination in the Filamentous Fungus Penicillium Roqueforti}, author = { C. Gil-Duran and J.F. Rojas-Aedo and E. Medina and I. Vaca and R.O. Garcia-Rico and S. Villagran and G. Levican and R. Chavez}, url = {/brokenurl#<Go to ISI>://WOS:000356353700059}, doi = {10.1371/journal.pone.0120740}, issn = {1932-6203}, year = {2015}, date = {2015-01-01}, journal = {Plos One}, volume = {10}, number = {3}, abstract = {Proteins containing Zn(II)(2)Cys(6) domains are exclusively found in fungi and yeasts. Genes encoding this class of proteins are broadly distributed in fungi, but few of them have been functionally characterized. In this work, we have characterized a gene from the filamentous fungus Penicillium roqueforti that encodes a Zn(II)(2)Cys(6) protein, whose function to date remains unknown. We have named this gene pcz1. We showed that the expression of pcz1 is negatively regulated in a P. roqueforti strain containing a dominant active Gai protein, suggesting that pcz1 encodes a downstream effector that is negatively controlled by Gai. More interestingly, the silencing of pcz1 in P. roqueforti using RNAi-silencing technology resulted in decreased apical growth, the promotion of conidial germination (even in the absence of a carbon source), and the strong repression of conidiation, concomitant with the downregulation of the genes of the central conidiation pathway brlA, abaA and wetA. A model for the participation of pcz1 in these physiological processes in P. roqueforti is proposed.}, keywords = {alpha-subunit, aspergillus-nidulans, chrysogenum, cluster, pga1}, pubstate = {published}, tppubtype = {article} } Proteins containing Zn(II)(2)Cys(6) domains are exclusively found in fungi and yeasts. Genes encoding this class of proteins are broadly distributed in fungi, but few of them have been functionally characterized. In this work, we have characterized a gene from the filamentous fungus Penicillium roqueforti that encodes a Zn(II)(2)Cys(6) protein, whose function to date remains unknown. We have named this gene pcz1. We showed that the expression of pcz1 is negatively regulated in a P. roqueforti strain containing a dominant active Gai protein, suggesting that pcz1 encodes a downstream effector that is negatively controlled by Gai. More interestingly, the silencing of pcz1 in P. roqueforti using RNAi-silencing technology resulted in decreased apical growth, the promotion of conidial germination (even in the absence of a carbon source), and the strong repression of conidiation, concomitant with the downregulation of the genes of the central conidiation pathway brlA, abaA and wetA. A model for the participation of pcz1 in these physiological processes in P. roqueforti is proposed. |
2017 |
The Biosynthetic Gene Cluster for Andrastin a in Penicillium Roqueforti Artículo de revista Frontiers in Microbiology, 8 , 2017, ISSN: 1664-302x. |
2016 |
Raman and Surface Enhanced Raman Scattering Study of the Orientation of Cruciform 9,10-Anthracene Thiophene and Furan Derivatives Deposited on a Gold Colloidal Surface Artículo de revista Journal of Molecular Structure, 1122 , pp. 198-204, 2016, ISSN: 0022-2860. |
2015 |
The Pcz1 Gene, Which Encodes a Zn(Ii)(2)Cys(6) Protein, Is Involved in the Control of Growth, Conidiation, and Conidial Germination in the Filamentous Fungus Penicillium Roqueforti Artículo de revista Plos One, 10 (3), 2015, ISSN: 1932-6203. |