Publicaciones 2011-2020

@article{RN338,
title = {Role of Sfk1 Gene in the Filamentous Fungus Penicillium Roqueforti},
author = { C. Torrent and C. Gil-Duran and J.F. Rojas-Aedo and E. Medina and I. Vaca and P. Castro and R.O. Garcia-Rico and M. Cotoras and L. Mendoza and G. Levican and R. Chavez},
url = {/brokenurl#<Go to ISI>://WOS:000417142700001},
doi = {10.3389/fmicb.2017.02424},
issn = {1664-302x},
year = {2017},
date = {2017-01-01},
journal = {Frontiers in Microbiology},
volume = {8},
abstract = {The sfk1 (suppressor of four kinase) gene has been mainly studied in Saccharomyces cerevisiae, where it was shown to be involved in growth and thermal stress resistance. This gene is widely conserved within the phylum Ascomycota. Despite this, to date sfk1 has not been studied in any filamentous fungus. Previously, we found that the orthologous of sfk1 was differentially expressed in a strain of Penicillium roqueforti with an altered phenotype. In this work, we have performed a functional characterization of this gene by using RNAi-silencing technology. The silencing of sfk1 in P. roqueforti resulted in decreased apical growth and the promotion of conidial germination, but interesting, it had no effect on conidiation. In addition, the attenuation of the sfk1 expression sensitized the fungus to osmotic stress, but not to thermal stress. RNA-mediated gene-silencing of sfk1 also affected cell wall integrity in the fungus. Finally, the silencing of sfk1 depleted the production of the main secondary metabolites of P. roqueforti, namely roquefortine C, andrastin A, and mycophenolic acid. To the best of our knowledge this is the first study of the sfk1 gene in filamentous fungi.},
keywords = {alpha-subunit, biosynthesis, botrytis-cinerea, changes, chrysogenum, expression, four gene germination, growth kinase, metabolites, of pathways, penicillium phenotypic protein rna-mediated roqueforti, saccharomyces-cerevisiae, secondary signaling silencing, stress, suppressor},
pubstate = {published},
tppubtype = {article}
}

@article{RN339,
title = {Heterotrimeric G Protein Alpha Subunit Controls Growth, Stress Response, Extracellular Protease Activity, and Cyclopiazonic Acid Production in Penicillium Camemberti},
author = { R.O. Garcia-Rico and C. Gil-Duran and J.F. Rojas-Aedo and I. Vaca and L. Figueroa and G. Levican and R. Chavez},
url = {/brokenurl#<Go to ISI>://WOS:000419930600002},
doi = {10.1016/j.funbio.2017.05.007},
issn = {1878-6146},
year = {2017},
date = {2017-01-01},
journal = {Fungal Biology},
volume = {121},
number = {9},
pages = {754-762},
publisher = {2017 British Mycological Society. Published by Elsevier Ltd.},
abstract = {The fungus Penicillium camemberti is widely used in the ripening of various bloomy-rind cheeses. Several properties of P. camemberti are important in cheese ripening, including conidiation, growth and enzyme production, among others. However, the production of mycotoxins such as cyclopiazonic acid during the ripening process by P. camemberti has raised concerns among consumers that demand food with minimal contamination. Here we show that overexpressing an alpha-subunit from the subgroup I of the heterotrimeric G protein (G alpha i) influences several of these processes: it negatively affects growth in a media dependent manner, triggers conidial germination, reduces the rate of sporulation, affects thermal and osmotic stress resistance, and also extracellular protease and cyclopiazonic acid production. Our results contribute to understanding the biological determinants underlying these biological processes in the economically important fungus P. camemberti.},
keywords = {alpha-subunit, aspergillus-nidulans, expression, filamentous fungus, g-beta, gene, geotrichum-candidum, growth, metabolism, micotoxin, mycotoxin pathogenicity, pcr, production, proteases, real-time reproduction resistance, secondary stress vegetative},
pubstate = {published},
tppubtype = {article}
}

@article{RN240,
title = {The Pcz1 Gene, Which Encodes a Zn(Ii)(2)Cys(6) Protein, Is Involved in the Control of Growth, Conidiation, and Conidial Germination in the Filamentous Fungus Penicillium Roqueforti},
author = { C. Gil-Duran and J.F. Rojas-Aedo and E. Medina and I. Vaca and R.O. Garcia-Rico and S. Villagran and G. Levican and R. Chavez},
url = {/brokenurl#<Go to ISI>://WOS:000356353700059},
doi = {10.1371/journal.pone.0120740},
issn = {1932-6203},
year = {2015},
date = {2015-01-01},
journal = {Plos One},
volume = {10},
number = {3},
abstract = {Proteins containing Zn(II)(2)Cys(6) domains are exclusively found in fungi and yeasts. Genes encoding this class of proteins are broadly distributed in fungi, but few of them have been functionally characterized. In this work, we have characterized a gene from the filamentous fungus Penicillium roqueforti that encodes a Zn(II)(2)Cys(6) protein, whose function to date remains unknown. We have named this gene pcz1. We showed that the expression of pcz1 is negatively regulated in a P. roqueforti strain containing a dominant active Gai protein, suggesting that pcz1 encodes a downstream effector that is negatively controlled by Gai. More interestingly, the silencing of pcz1 in P. roqueforti using RNAi-silencing technology resulted in decreased apical growth, the promotion of conidial germination (even in the absence of a carbon source), and the strong repression of conidiation, concomitant with the downregulation of the genes of the central conidiation pathway brlA, abaA and wetA. A model for the participation of pcz1 in these physiological processes in P. roqueforti is proposed.},
keywords = {alpha-subunit, aspergillus-nidulans, chrysogenum, cluster, pga1},
pubstate = {published},
tppubtype = {article}
}