Publicaciones 2011-2020

@article{RN389,
title = {Dark Classics in Chemical Neuroscience: Mescaline},
author = { B.K. Cassels and P. Saez-Briones},
url = {/brokenurl#<Go to ISI>://WOS:000447954300015},
doi = {10.1021/acschemneuro.8b00215},
issn = {1948-7193},
year = {2018},
date = {2018-01-01},
journal = {Acs Chemical Neuroscience},
volume = {9},
number = {10},
pages = {2448-2458},
abstract = {Archeological studies in the United States, Mexico, and Peru suggest that mescaline, as a cactus constituent, has been used for more than 6000 years. Although it is a widespread cactus alkaloid, it is present in high concentrations in few species, notably the North American peyote (Lophophora williamsii) and the South American wachuma (Trichocereus pachanoi, T. peruvianus, and T. bridgesii). Spanish 16th century chroniclers considered these cacti "diabolic", leading to their prohibition, but their use persisted to our days and has been spreading for the last 150 years. In the late 1800s, peyote attracted scientific attention; mescaline was isolated, and its role in the psychedelic effects of peyote tops or "mescal buttons" was demonstrated. Its structure was established by synthesis in 1929, and alternative routes were developed, providing larger amounts for pharmacological and biosynthetic research. Although its effects are attributed mainly to its action as a 5-HT2A serotonin receptor agonist, mescaline binds in a similar concentration range to 5-HT1A and alpha(2A) receptors. It is largely excreted unchanged in human urine, and its metabolic products are apparently unrelated to its psychedelic properties. Its low potency is probably responsible for its relative neglect by recreational substance users, as the successful search for structure-activity relationships in the hallucinogen field focused largely on finding more potent analogues. Renewed interest in the possible therapeutic applications of psychedelic drugs may hopefully lead to novel insights regarding the commonalities and differences between the actions of individual classic hallucinogens.},
keywords = {analogs, biosynthesis, cancer, derivatives, diethylamide, hallucinogen, hallucinogenic life-threatening lysergic-acid mescaline, metabolism, pedro, peyote, pharmacology, phenethylamine, phenyl-aethylamine, properties, relationships, san serotonin stimulus structure-activity synthesis, wachuma},
pubstate = {published},
tppubtype = {article}
}

@article{RN382,
title = {The Developmental Regulator Pcz1 Affects the Production of Secondary Metabolites in the Filamentous Fungus Penicillium Roqueforti},
author = { J.F. Rojas-Aedo and C. Gil-Duran and A. Goity and I. Vaca and G. Levican and L.F. Larrondo and R. Chavez},
url = {/brokenurl#<Go to ISI>://WOS:000438320100007},
doi = {10.1016/j.micres.2018.05.005},
issn = {0944-5013},
year = {2018},
date = {2018-01-01},
journal = {Microbiological Research},
volume = {212},
pages = {67-74},
abstract = {Penicillium roqueforti is used in the production of several kinds of ripened blue-veined cheeses. In addition, this fungus produces interesting secondary metabolites such as roquefortine C, andrastin A and mycophenolic acid. To date, there is scarce information concerning the regulation of the production of these secondary metabolites. Recently, the gene named pcz1 (Penicillium C6 zinc domain protein 1) was described in P. roqueforti, which encodes for a Zn(II)(2)Cys(6) protein that controls growth and developmental processes in this fungus. However, its effect on secondary metabolism is currently unknown. In this work, we have analyzed how the overexpression and down-regulation of pcz1 affect the production of roquefortine C, andrastin A and mycophenolic acid in P. roqueforti. The three metabolites were drastically reduced in the pcz1 down-regulated strains. However, when pcz1 was overexpressed, only mycophenolic acid was overproduced while, on the contrary, levels of roquefortine C and andrastin A were diminished. Importantly, these results match the expression pattern of keywords genes involved in the biosynthesis of these metabolites. Taken together, our results suggest that Pcz1 plays a keywords role in regulating secondary metabolism in the fungus Penicillium roqueforti.},
keywords = {biosynthesis, chrysogenum complex, cross-talk, discovery, expression, gene-cluster, laea, metabolism, mycophenolic-acid, pathway pcz1, penicillium protein, roqueforti, secondary subunit},
pubstate = {published},
tppubtype = {article}
}

@article{RN339,
title = {Heterotrimeric G Protein Alpha Subunit Controls Growth, Stress Response, Extracellular Protease Activity, and Cyclopiazonic Acid Production in Penicillium Camemberti},
author = { R.O. Garcia-Rico and C. Gil-Duran and J.F. Rojas-Aedo and I. Vaca and L. Figueroa and G. Levican and R. Chavez},
url = {/brokenurl#<Go to ISI>://WOS:000419930600002},
doi = {10.1016/j.funbio.2017.05.007},
issn = {1878-6146},
year = {2017},
date = {2017-01-01},
journal = {Fungal Biology},
volume = {121},
number = {9},
pages = {754-762},
publisher = {2017 British Mycological Society. Published by Elsevier Ltd.},
abstract = {The fungus Penicillium camemberti is widely used in the ripening of various bloomy-rind cheeses. Several properties of P. camemberti are important in cheese ripening, including conidiation, growth and enzyme production, among others. However, the production of mycotoxins such as cyclopiazonic acid during the ripening process by P. camemberti has raised concerns among consumers that demand food with minimal contamination. Here we show that overexpressing an alpha-subunit from the subgroup I of the heterotrimeric G protein (G alpha i) influences several of these processes: it negatively affects growth in a media dependent manner, triggers conidial germination, reduces the rate of sporulation, affects thermal and osmotic stress resistance, and also extracellular protease and cyclopiazonic acid production. Our results contribute to understanding the biological determinants underlying these biological processes in the economically important fungus P. camemberti.},
keywords = {alpha-subunit, aspergillus-nidulans, expression, filamentous fungus, g-beta, gene, geotrichum-candidum, growth, metabolism, micotoxin, mycotoxin pathogenicity, pcr, production, proteases, real-time reproduction resistance, secondary stress vegetative},
pubstate = {published},
tppubtype = {article}
}