2017 |
Hugo, E A; Cassels, B K; Fierro, A Functional Roles of T3.37 and S5.46 in the Activation Mechanism of the Dopamine D1 Receptor Artículo de revista Journal of Molecular Modeling, 23 (4), 2017, ISSN: 1610-2940. Resumen | Enlaces | BibTeX | Etiquetas: crystal-structure, d-1, d1 dopamine dopamine, dynamics, identification, interactions, models, molecular pharmacophore, receptor, recognition residues, s5.46, serine structures @article{hugo2017functional, title = {Functional Roles of T3.37 and S5.46 in the Activation Mechanism of the Dopamine D1 Receptor}, author = { E.A. Hugo and B.K. Cassels and A. Fierro}, url = {/brokenurl#<Go to ISI>://WOS:000399406500014}, doi = {10.1007/s00894-017-3313-0}, issn = {1610-2940}, year = {2017}, date = {2017-01-01}, journal = {Journal of Molecular Modeling}, volume = {23}, number = {4}, abstract = {The activation mechanism of dopamine receptors is unknown. The amino acids S5.42, S5.43, and S5.46 located in helix 5 appear to be crucial, but their specific roles in receptor activation have not been studied. We modeled the D1 dopamine receptor using the crystal structures of the D3 dopamine and beta 2 adrenergic receptors. Molecular dynamics simulations show that the interaction of dopamine with the D1 receptor leads to the formation of a hydrogen-bond network with its catechol group and helices 3, 5, and 6, including water molecules. The para hydroxyl group of dopamine binds directly to S5.42 and N6.55, the latter also interacting with S5.43. Unexpectedly, S5.46 does not interact directly with the catechol; instead, it interacts through a water molecule with S5.42 and directly with T3.37. The formation of this hydrogen-bond network, part of which was previously observed in docking studies with dopamine agonists, triggers the opening of the E6.30-R3.60 ionic lock associated with the activation of GPCRs. These changes do not occur in the unbonded (apo) receptor or when it is in a complex with the antagonist 3-methoxy- 5,6,7,8,9,14-hexahydrodibenz[d, g]azecine. Our results provide valuable insight into the T3.37-S5.46-water-S5.43-ligand interaction, which may be crucial to the activation of the D1 dopamine receptor and should be considered during the design of novel agonists.}, keywords = {crystal-structure, d-1, d1 dopamine dopamine, dynamics, identification, interactions, models, molecular pharmacophore, receptor, recognition residues, s5.46, serine structures}, pubstate = {published}, tppubtype = {article} } The activation mechanism of dopamine receptors is unknown. The amino acids S5.42, S5.43, and S5.46 located in helix 5 appear to be crucial, but their specific roles in receptor activation have not been studied. We modeled the D1 dopamine receptor using the crystal structures of the D3 dopamine and beta 2 adrenergic receptors. Molecular dynamics simulations show that the interaction of dopamine with the D1 receptor leads to the formation of a hydrogen-bond network with its catechol group and helices 3, 5, and 6, including water molecules. The para hydroxyl group of dopamine binds directly to S5.42 and N6.55, the latter also interacting with S5.43. Unexpectedly, S5.46 does not interact directly with the catechol; instead, it interacts through a water molecule with S5.42 and directly with T3.37. The formation of this hydrogen-bond network, part of which was previously observed in docking studies with dopamine agonists, triggers the opening of the E6.30-R3.60 ionic lock associated with the activation of GPCRs. These changes do not occur in the unbonded (apo) receptor or when it is in a complex with the antagonist 3-methoxy- 5,6,7,8,9,14-hexahydrodibenz[d, g]azecine. Our results provide valuable insight into the T3.37-S5.46-water-S5.43-ligand interaction, which may be crucial to the activation of the D1 dopamine receptor and should be considered during the design of novel agonists. |
Nett, R S; Montanares, M; Marcassa, A; Lul, X; Nagel, R; Charles, T C; Hedden, P; Rojas, M C; Peters, R J Elucidation of Gibberellin Biosynthesis in Bacteria Reveals Convergent Evolution Artículo de revista Nature Chemical Biology, 13 (1), pp. 69-74, 2017, ISSN: 1552-4450. Resumen | Enlaces | BibTeX | Etiquetas: artemisinin biosynthesis, bradyrhizobium-japonicum, cloning, cytochrome-p450 ent-kaurene, fungi, gene-cluster, green-revolution, identification, plants, rearrangement @article{RN347, title = {Elucidation of Gibberellin Biosynthesis in Bacteria Reveals Convergent Evolution}, author = { R.S. Nett and M. Montanares and A. Marcassa and X. Lul and R. Nagel and T.C. Charles and P. Hedden and M.C. Rojas and R.J. Peters}, url = {/brokenurl#<Go to ISI>://WOS:000393267200015}, doi = {10.1038/Nchembio.2232}, issn = {1552-4450}, year = {2017}, date = {2017-01-01}, journal = {Nature Chemical Biology}, volume = {13}, number = {1}, pages = {69-74}, abstract = {Gibberellins (GAs) are crucial phytohormones involved in many aspects of plant growth and development, including plant-microbe interactions, which has led to GA production by plant-associated fungi and bacteria as well. While the GA biosynthetic pathways in plants and fungi have been elucidated and found to have arisen independently through convergent evolution, little has been uncovered about GA biosynthesis in bacteria. Some nitrogen-fixing, symbiotic, legume-associated rhizobia, including Bradyrhizobium japonicum-the symbiont of soybean and Sinorhizobium fredii-a broad-host-nodulating species-contain a putative GA biosynthetic operon, or gene cluster. Through functional characterization of five unknown genes, we demonstrate that this operon encodes the enzymes necessary to produce GA(9), thereby elucidating bacterial GA biosynthesis. The distinct nature of these enzymes indicates that bacteria have independently evolved a third biosynthetic pathway for GA production. Furthermore, our results also reveal a central biochemical logic that is followed in all three convergently evolved GA biosynthetic pathways.}, keywords = {artemisinin biosynthesis, bradyrhizobium-japonicum, cloning, cytochrome-p450 ent-kaurene, fungi, gene-cluster, green-revolution, identification, plants, rearrangement}, pubstate = {published}, tppubtype = {article} } Gibberellins (GAs) are crucial phytohormones involved in many aspects of plant growth and development, including plant-microbe interactions, which has led to GA production by plant-associated fungi and bacteria as well. While the GA biosynthetic pathways in plants and fungi have been elucidated and found to have arisen independently through convergent evolution, little has been uncovered about GA biosynthesis in bacteria. Some nitrogen-fixing, symbiotic, legume-associated rhizobia, including Bradyrhizobium japonicum-the symbiont of soybean and Sinorhizobium fredii-a broad-host-nodulating species-contain a putative GA biosynthetic operon, or gene cluster. Through functional characterization of five unknown genes, we demonstrate that this operon encodes the enzymes necessary to produce GA(9), thereby elucidating bacterial GA biosynthesis. The distinct nature of these enzymes indicates that bacteria have independently evolved a third biosynthetic pathway for GA production. Furthermore, our results also reveal a central biochemical logic that is followed in all three convergently evolved GA biosynthetic pathways. |
Corales, G; Celis, F; Gomez-Jeria, J S; Campos-Vallette, M; Carcamo, J J Raman of Indigo on a Silver Surface. Raman and Theoretical Characterization of Indigo Deposited on Silicon Dioxide-Coated and Uncoated Silver Nanoparticles Artículo de revista Spectroscopy Letters, 50 (6), pp. 316-321, 2017, ISSN: 0038-7010. Resumen | Enlaces | BibTeX | Etiquetas: analysis, approximations, dioxide-coated fluorescence, identification, indigo, molecular-orbital nanoparticles, raman, scattering, sers sers, shiners, silicon silver spectroscopic theories @article{RN378, title = {Raman of Indigo on a Silver Surface. Raman and Theoretical Characterization of Indigo Deposited on Silicon Dioxide-Coated and Uncoated Silver Nanoparticles}, author = { G. Corales and F. Celis and J.S. Gomez-Jeria and M. Campos-Vallette and J.J. Carcamo}, url = {/brokenurl#<Go to ISI>://WOS:000404931300004}, doi = {10.1080/00387010.2017.1324493}, issn = {0038-7010}, year = {2017}, date = {2017-01-01}, journal = {Spectroscopy Letters}, volume = {50}, number = {6}, pages = {316-321}, abstract = {Raman, surface-enhanced Raman scattering, and shell isolated nanoparticles-enhanced Raman scattering techniques were used to study the indigo-nanoparticle interaction nature. Silver nanoparticles were employed with and without a silicon dioxide spacer inert layer. The SERS spectral profile, obtained using silver nanoparticles, is different from the Raman one, which led to the proposition that the indigo-silver interaction is in the range of intermolecular interactions. SERS spectral reproducibility suggests identical organization and orientation of the analyte on the metal surface. The shell isolated nanoparticles enhanced Raman scattering spectrum of indigo, obtained by using silicon dioxide coated silver nanoparticles resulted similar to its Raman spectrum. This result indicates that the indigo structure is chemically unmodified by the silicon dioxide-coated silver surface. From the shell-isolated nanoparticles-enhanced Raman scattering experiments, the electromagnetic mechanism is proposed as the reason for the spectral enhancement. Theoretical calculations allow one to infer both the indigo-silver surface interaction nature and the orientation of indigo on the surface.}, keywords = {analysis, approximations, dioxide-coated fluorescence, identification, indigo, molecular-orbital nanoparticles, raman, scattering, sers sers, shiners, silicon silver spectroscopic theories}, pubstate = {published}, tppubtype = {article} } Raman, surface-enhanced Raman scattering, and shell isolated nanoparticles-enhanced Raman scattering techniques were used to study the indigo-nanoparticle interaction nature. Silver nanoparticles were employed with and without a silicon dioxide spacer inert layer. The SERS spectral profile, obtained using silver nanoparticles, is different from the Raman one, which led to the proposition that the indigo-silver interaction is in the range of intermolecular interactions. SERS spectral reproducibility suggests identical organization and orientation of the analyte on the metal surface. The shell isolated nanoparticles enhanced Raman scattering spectrum of indigo, obtained by using silicon dioxide coated silver nanoparticles resulted similar to its Raman spectrum. This result indicates that the indigo structure is chemically unmodified by the silicon dioxide-coated silver surface. From the shell-isolated nanoparticles-enhanced Raman scattering experiments, the electromagnetic mechanism is proposed as the reason for the spectral enhancement. Theoretical calculations allow one to infer both the indigo-silver surface interaction nature and the orientation of indigo on the surface. |
2015 |
Campos-Vallette, M; Aguayo, T Vibrational Spectroscopy for the Study of Chilean Cultural Heritage Artículo de revista Heritage Science, 3 , 2015, ISSN: 2050-7445. Resumen | Enlaces | BibTeX | Etiquetas: art, enhanced ft-raman, identification, iron-oxides, micro-raman, microspectroscopy paintings, pigments, raman-scattering, spectra @article{RN263, title = {Vibrational Spectroscopy for the Study of Chilean Cultural Heritage}, author = { M. Campos-Vallette and T. Aguayo}, url = {/brokenurl#<Go to ISI>://WOS:000356922100001}, doi = {10.1186/s40494-015-0047-0}, issn = {2050-7445}, year = {2015}, date = {2015-01-01}, journal = {Heritage Science}, volume = {3}, abstract = {Background: Natural and synthetic colouring products along with its associated supporting materials have been studied in several expressions of the Chilean cultural heritage using vibrational spectroscopy. These expressions include archaeological remains as well as works of art., Results: Among the materials studied so far we can count pigments found in archaeological sites from the north of Chile, in plaster (wall paintings) and in polychrome (beams), and dyes mainly related to added components on historic silk textiles. Identification of materials resulted to be relevant to know about Chilean culture through history., Conclusions: The vibrational knowledge obtained contributes to give solid data as a complement to the different information aspects collected by conservation professionals involved in the characterization and conservation procedures of cultural heritage and also it gives us the opportunity to share knowledge and to give value to objects that not always are of the public domain.}, keywords = {art, enhanced ft-raman, identification, iron-oxides, micro-raman, microspectroscopy paintings, pigments, raman-scattering, spectra}, pubstate = {published}, tppubtype = {article} } Background: Natural and synthetic colouring products along with its associated supporting materials have been studied in several expressions of the Chilean cultural heritage using vibrational spectroscopy. These expressions include archaeological remains as well as works of art., Results: Among the materials studied so far we can count pigments found in archaeological sites from the north of Chile, in plaster (wall paintings) and in polychrome (beams), and dyes mainly related to added components on historic silk textiles. Identification of materials resulted to be relevant to know about Chilean culture through history., Conclusions: The vibrational knowledge obtained contributes to give solid data as a complement to the different information aspects collected by conservation professionals involved in the characterization and conservation procedures of cultural heritage and also it gives us the opportunity to share knowledge and to give value to objects that not always are of the public domain. |
2014 |
Henriquez, M; Vergara, K; Norambuena, J; Beiza, A; Maza, F; Ubilla, P; Araya, I; Chavez, R; San-Martin, A; Darias, J; Darias, M J; Vaca, I Diversity of Cultivable Fungi Associated with Antarctic Marine Sponges and Screening for Their Antimicrobial, Antitumoral and Antioxidant Potential Artículo de revista World Journal of Microbiology & Biotechnology, 30 (1), pp. 65-76, 2014, ISSN: 0959-3993. Resumen | Enlaces | BibTeX | Etiquetas: antarctic antimicrobial, antioxidant, antitumoral, bioactive coastal diversity, fungi, geomyces haliclona-simulans, identification, metabolites, microorganisms natural-products, penicillium, phylogenetic sp., sponges, suberites-zeteki, syndrome, waters, white-nose @article{RN180, title = {Diversity of Cultivable Fungi Associated with Antarctic Marine Sponges and Screening for Their Antimicrobial, Antitumoral and Antioxidant Potential}, author = { M. Henriquez and K. Vergara and J. Norambuena and A. Beiza and F. Maza and P. Ubilla and I. Araya and R. Chavez and A. San-Martin and J. Darias and M.J. Darias and I. Vaca}, url = {/brokenurl#<Go to ISI>://WOS:000329248200007}, doi = {10.1007/s11274-013-1418-x}, issn = {0959-3993}, year = {2014}, date = {2014-01-01}, journal = {World Journal of Microbiology & Biotechnology}, volume = {30}, number = {1}, pages = {65-76}, abstract = {The diversity of sponge-associated fungi has been poorly investigated in remote geographical areas like Antarctica. In this study, 101 phenotypically different fungal isolates were obtained from 11 sponge samples collected in King George Island, Antarctica. The analysis of ITS sequences revealed that they belong to the phylum Ascomycota. Sixty-five isolates belong to the genera Geomyces, Penicillium, Epicoccum, Pseudeurotium, Thelebolus, Cladosporium, Aspergillus, Aureobasidium, Phoma, and Trichocladium but 36 isolates could not be identified at genus level. In order to estimate the potential of these isolates as producers of interesting bioactivities, antimicrobial, antitumoral and antioxidant activities of fungal culture extracts were assayed. Around 51 % of the extracts, mainly from the genus Geomyces and non identified relatives, showed antimicrobial activity against some of the bacteria tested. On the other hand, around 42 % of the extracts showed potent antitumoral activity, Geomyces sp. having the best performance. Finally, the potential of the isolated fungi as producers of antioxidant activity seems to be moderate. Our results suggest that fungi associated with Antarctic sponges, particularly Geomyces, would be valuable sources of antimicrobial and antitumoral compounds. To our knowledge, this is the first report describing the biodiversity and the metabolic potential of fungi associated with Antarctic marine sponges.}, keywords = {antarctic antimicrobial, antioxidant, antitumoral, bioactive coastal diversity, fungi, geomyces haliclona-simulans, identification, metabolites, microorganisms natural-products, penicillium, phylogenetic sp., sponges, suberites-zeteki, syndrome, waters, white-nose}, pubstate = {published}, tppubtype = {article} } The diversity of sponge-associated fungi has been poorly investigated in remote geographical areas like Antarctica. In this study, 101 phenotypically different fungal isolates were obtained from 11 sponge samples collected in King George Island, Antarctica. The analysis of ITS sequences revealed that they belong to the phylum Ascomycota. Sixty-five isolates belong to the genera Geomyces, Penicillium, Epicoccum, Pseudeurotium, Thelebolus, Cladosporium, Aspergillus, Aureobasidium, Phoma, and Trichocladium but 36 isolates could not be identified at genus level. In order to estimate the potential of these isolates as producers of interesting bioactivities, antimicrobial, antitumoral and antioxidant activities of fungal culture extracts were assayed. Around 51 % of the extracts, mainly from the genus Geomyces and non identified relatives, showed antimicrobial activity against some of the bacteria tested. On the other hand, around 42 % of the extracts showed potent antitumoral activity, Geomyces sp. having the best performance. Finally, the potential of the isolated fungi as producers of antioxidant activity seems to be moderate. Our results suggest that fungi associated with Antarctic sponges, particularly Geomyces, would be valuable sources of antimicrobial and antitumoral compounds. To our knowledge, this is the first report describing the biodiversity and the metabolic potential of fungi associated with Antarctic marine sponges. |
Araya, C; Jaque, J; Naranjo, N; Icaza, M; Clavijo, E; Aguayo, T; Campos-Vallette, M Raman Characterization of Pigments in Painted Beams and a Wall Painting Discovered in the San Francisco Church in Santiago, Chile Artículo de revista Spectroscopy Letters, 47 (3), pp. 177-183, 2014, ISSN: 0038-7010. Resumen | Enlaces | BibTeX | Etiquetas: analyses, binding copper, corrosion, ft-raman, identification, lead library, media, microchemical minerals, mural painting, polychrome, raman spectra, spectroscopy, wood @article{RN211, title = {Raman Characterization of Pigments in Painted Beams and a Wall Painting Discovered in the San Francisco Church in Santiago, Chile}, author = { C. Araya and J. Jaque and N. Naranjo and M. Icaza and E. Clavijo and T. Aguayo and M. Campos-Vallette}, url = {/brokenurl#<Go to ISI>://WOS:000329200400003}, doi = {10.1080/00387010.2013.788521}, issn = {0038-7010}, year = {2014}, date = {2014-01-01}, journal = {Spectroscopy Letters}, volume = {47}, number = {3}, pages = {177-183}, abstract = {A material characterization of two artworks discovered in the San Francisco Church, Santiago, Chile, was performed using micro-Raman spectroscopy. Structural painted beams and a wall painting that belong to the same time period, between the end of the 17th and 19th centuries, were analyzed. The cross-section samples of both artworks were characterized and animal protein was identified in the ground layer in both cases. The supporting material of the beams was identified as cypress wood, and a rag paper layer was used as a base for the paint layer, which is composed mainly of a white ground layer on which the color was subsequently added; the yellow pigments are orpiment and chrome yellow; the green color probably arises from a mixture of orpiment, red lead, ultramarine blue, and calcite. A complete analysis of the materials using complementary techniques such as microchemistry and optical microscopy indicates that the mural was painted using a mixed technique and that organic and inorganic pigments were used. The identification of the synthetic pigment ultramarine blue in some blue areas of the wall revealed a modification of the wall painting in the 19th century; dark blue areas resulted from a mixture of indigo, palygorskite, and lazurite.}, keywords = {analyses, binding copper, corrosion, ft-raman, identification, lead library, media, microchemical minerals, mural painting, polychrome, raman spectra, spectroscopy, wood}, pubstate = {published}, tppubtype = {article} } A material characterization of two artworks discovered in the San Francisco Church, Santiago, Chile, was performed using micro-Raman spectroscopy. Structural painted beams and a wall painting that belong to the same time period, between the end of the 17th and 19th centuries, were analyzed. The cross-section samples of both artworks were characterized and animal protein was identified in the ground layer in both cases. The supporting material of the beams was identified as cypress wood, and a rag paper layer was used as a base for the paint layer, which is composed mainly of a white ground layer on which the color was subsequently added; the yellow pigments are orpiment and chrome yellow; the green color probably arises from a mixture of orpiment, red lead, ultramarine blue, and calcite. A complete analysis of the materials using complementary techniques such as microchemistry and optical microscopy indicates that the mural was painted using a mixed technique and that organic and inorganic pigments were used. The identification of the synthetic pigment ultramarine blue in some blue areas of the wall revealed a modification of the wall painting in the 19th century; dark blue areas resulted from a mixture of indigo, palygorskite, and lazurite. |
2013 |
Sepulveda, M; Gutierrez, S; Campos-Vallette, M; Clavijo, E; Walter, P; Carcamo, J J Raman Spectroscopy and X-Ray Fluorescence in Molecular Analysis of Yellow Blocks from the Archeological Site Playa Miller 7 (Northern Chile) Artículo de revista Journal of the Chilean Chemical Society, 58 (3), pp. 1836-1839, 2013, ISSN: 0717-9707. Resumen | Enlaces | BibTeX | Etiquetas: art, chile, diagnosis, fluorescence, identification, jarosite, micro-raman, northern pigments, raman spectra spectroscopy, x-ray @article{RN152, title = {Raman Spectroscopy and X-Ray Fluorescence in Molecular Analysis of Yellow Blocks from the Archeological Site Playa Miller 7 (Northern Chile)}, author = { M. Sepulveda and S. Gutierrez and M. Campos-Vallette and E. Clavijo and P. Walter and J.J. Carcamo}, url = {/brokenurl#<Go to ISI>://WOS:000331238200008}, doi = {10.4067/S0717-97072013000300008}, issn = {0717-9707}, year = {2013}, date = {2013-01-01}, journal = {Journal of the Chilean Chemical Society}, volume = {58}, number = {3}, pages = {1836-1839}, abstract = {Yellow blocks from the archaeological site Playa Miller 7 (PLM7), on the coast of Atacama Desert in northern Chile, were analyzed by Raman spectroscopy and X-ray fluorescence (XRF) portable. Our results identify for the first time the use of K-jarosite and natrojarosite in prehispanic times (approx. 2500 year BP). In search of a possible source of supply for this mineral hydrothermal origin, our surveys were focused on Andean geothermal areas with identification, so far, from a single source in the region of Arica and Parinacota: Jurasi (JU), located at 4000 mamsl. Comparison of the Raman spectra between samples archaeological and Jurasi, allow us to infer that this hydrothermal source could be used as obtaining source of yellow pigment by prehispanic inhabitant of Formative period (3700-1500 years B.P.).}, keywords = {art, chile, diagnosis, fluorescence, identification, jarosite, micro-raman, northern pigments, raman spectra spectroscopy, x-ray}, pubstate = {published}, tppubtype = {article} } Yellow blocks from the archaeological site Playa Miller 7 (PLM7), on the coast of Atacama Desert in northern Chile, were analyzed by Raman spectroscopy and X-ray fluorescence (XRF) portable. Our results identify for the first time the use of K-jarosite and natrojarosite in prehispanic times (approx. 2500 year BP). In search of a possible source of supply for this mineral hydrothermal origin, our surveys were focused on Andean geothermal areas with identification, so far, from a single source in the region of Arica and Parinacota: Jurasi (JU), located at 4000 mamsl. Comparison of the Raman spectra between samples archaeological and Jurasi, allow us to infer that this hydrothermal source could be used as obtaining source of yellow pigment by prehispanic inhabitant of Formative period (3700-1500 years B.P.). |
Laich, F; Vaca, I; Chavez, R Rhodotorula Portillonensis Sp Nov., a Basidiomycetous Yeast Isolated from Antarctic Shallow-Water Marine Sediment Artículo de revista International Journal of Systematic and Evolutionary Microbiology, 63 , pp. 3884-3891, 2013, ISSN: 1466-5026. Resumen | Enlaces | BibTeX | Etiquetas: biodiversity, d1/d2 diversity, environments, identification, patagonia, psychrophilic rdna, systematics, yeasts @article{RN120, title = {Rhodotorula Portillonensis Sp Nov., a Basidiomycetous Yeast Isolated from Antarctic Shallow-Water Marine Sediment}, author = { F. Laich and I. Vaca and R. Chavez}, url = {/brokenurl#<Go to ISI>://WOS:000327910200056}, doi = {10.1099/ijs.0.052753-0}, issn = {1466-5026}, year = {2013}, date = {2013-01-01}, journal = {International Journal of Systematic and Evolutionary Microbiology}, volume = {63}, pages = {3884-3891}, abstract = {During the characterization of the mycobiota associated with shallow-water marine environments from Antarctic sea, a novel pink yeast species was isolated. Sequence analysis of the D1/D2 domain of the LSU rDNA gene and 5.8S-ITS regions revealed that the isolated yeast was closely related to Rhodotorula pallida CBS 320(T) and Rhodotorula benthica CBS 9124(T). On the basis of morphological, biochemical and physiological characterization and phylogenetic analyses, a novel basidiomycetous yeast species, Rhodotorula portillonensis sp. nov., is proposed. The type strain is Pi2(T) (=CBS 12733(T) =CECT 13081(T)) which was isolated from shallow-water marine sediment in Fildes Bay, King George Island, Antarctica.}, keywords = {biodiversity, d1/d2 diversity, environments, identification, patagonia, psychrophilic rdna, systematics, yeasts}, pubstate = {published}, tppubtype = {article} } During the characterization of the mycobiota associated with shallow-water marine environments from Antarctic sea, a novel pink yeast species was isolated. Sequence analysis of the D1/D2 domain of the LSU rDNA gene and 5.8S-ITS regions revealed that the isolated yeast was closely related to Rhodotorula pallida CBS 320(T) and Rhodotorula benthica CBS 9124(T). On the basis of morphological, biochemical and physiological characterization and phylogenetic analyses, a novel basidiomycetous yeast species, Rhodotorula portillonensis sp. nov., is proposed. The type strain is Pi2(T) (=CBS 12733(T) =CECT 13081(T)) which was isolated from shallow-water marine sediment in Fildes Bay, King George Island, Antarctica. |
2011 |
Aguayo, T; Clavijo, E; Eisner, F; Ossa-Izquierdo, C; Campos-Vallette, M Raman Spectroscopy in the Diagnosis of the Wall Painting History of Concepcion, Chile Artículo de revista Journal of Raman Spectroscopy, 42 (12), pp. 2143-2148, 2011, ISSN: 0377-0486. Resumen | Enlaces | BibTeX | Etiquetas: analysis, art, calcium, conservation, cultural-heritage, fluorescence ft-raman, identification, nondestructive of painting, pigment pigments, raman spectra, spectroscopy, wall work @article{RN40b, title = {Raman Spectroscopy in the Diagnosis of the Wall Painting History of Concepcion, Chile}, author = { T. Aguayo and E. Clavijo and F. Eisner and C. Ossa-Izquierdo and M. Campos-Vallette}, url = {/brokenurl#<Go to ISI>://WOS:000298581500013}, doi = {10.1002/jrs.2978}, issn = {0377-0486}, year = {2011}, date = {2011-01-01}, journal = {Journal of Raman Spectroscopy}, volume = {42}, number = {12}, pages = {2143-2148}, publisher = {2011 John Wiley & Sons, Ltd.}, abstract = {Pigments and fresco-related materials from the wall painting Historia de Concepcion by Gregorio De La Fuente in Concepcion, Chile were identified by Raman microscopy. The pigments were compared with those obtained from the artist's studio, and results allowed inference about subsequent interventions. Materials coming from processes such as salt efflorescence were also studied. The results contribute to the diagnosis of the current conservation state of the wall painting for its future restoration.}, keywords = {analysis, art, calcium, conservation, cultural-heritage, fluorescence ft-raman, identification, nondestructive of painting, pigment pigments, raman spectra, spectroscopy, wall work}, pubstate = {published}, tppubtype = {article} } Pigments and fresco-related materials from the wall painting Historia de Concepcion by Gregorio De La Fuente in Concepcion, Chile were identified by Raman microscopy. The pigments were compared with those obtained from the artist's studio, and results allowed inference about subsequent interventions. Materials coming from processes such as salt efflorescence were also studied. The results contribute to the diagnosis of the current conservation state of the wall painting for its future restoration. |