2014 |
Garcia-Beltran, O; Yanez, O; Caballero, J; Galdámez, A; Mena, N; Nunez, M; Cassels, B K Synthesis of Coumarin Derivatives as Fluorescent Probes for Membrane and Cell Dynamics Studies Artículo de revista European Journal of Medicinal Chemistry, 76 , pp. 79-86, 2014, ISSN: 0223-5234. Resumen | Enlaces | BibTeX | Etiquetas: biological-membranes, carboxylic-acids, cell coumarins, crystal-structure, decarboxylation depth, dynamics, energy-transfer, hydrogen-bond, membranes, model, molecular molecular-dynamics, probes, proteins, resonance @article{RN192, title = {Synthesis of Coumarin Derivatives as Fluorescent Probes for Membrane and Cell Dynamics Studies}, author = { O. Garcia-Beltran and O. Yanez and J. Caballero and A. Gald\'{a}mez and N. Mena and M. Nunez and B.K. Cassels}, url = {/brokenurl#<Go to ISI>://WOS:000335487400009}, doi = {10.1016/j.ejmech.2014.02.016}, issn = {0223-5234}, year = {2014}, date = {2014-01-01}, journal = {European Journal of Medicinal Chemistry}, volume = {76}, pages = {79-86}, publisher = {2014 Elsevier Masson SAS.}, abstract = {Three coumarin-derived fluorescent probes, 3-acetyl-7-[(6-bromohexyl)oxy]-2H-chromen-2-one (FM1), 7-[(6-bromohexyl)oxy]-4-methyl-2H-chromen-2-one (FM2) and ethyl 2-7-[(6-bromohexyl)oxy]-2-oxo-2H-chromen-4-ylacetate (FM3), are described, with their photophysical constants. The compounds were tested in preliminary studies employing epifluorescence microscopy demonstrating that they allow the imaging of human neuroblastoma SH-SY5Y cell membranes. The structure of FM3 was confirmed by X-ray crystallographic analysis. Molecular dynamics (MD) simulations were used to characterize the localization and interactions of the studied compounds with a lipid bilayer model of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC).}, keywords = {biological-membranes, carboxylic-acids, cell coumarins, crystal-structure, decarboxylation depth, dynamics, energy-transfer, hydrogen-bond, membranes, model, molecular molecular-dynamics, probes, proteins, resonance}, pubstate = {published}, tppubtype = {article} } Three coumarin-derived fluorescent probes, 3-acetyl-7-[(6-bromohexyl)oxy]-2H-chromen-2-one (FM1), 7-[(6-bromohexyl)oxy]-4-methyl-2H-chromen-2-one (FM2) and ethyl 2-7-[(6-bromohexyl)oxy]-2-oxo-2H-chromen-4-ylacetate (FM3), are described, with their photophysical constants. The compounds were tested in preliminary studies employing epifluorescence microscopy demonstrating that they allow the imaging of human neuroblastoma SH-SY5Y cell membranes. The structure of FM3 was confirmed by X-ray crystallographic analysis. Molecular dynamics (MD) simulations were used to characterize the localization and interactions of the studied compounds with a lipid bilayer model of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC). |
2014 |
Synthesis of Coumarin Derivatives as Fluorescent Probes for Membrane and Cell Dynamics Studies Artículo de revista European Journal of Medicinal Chemistry, 76 , pp. 79-86, 2014, ISSN: 0223-5234. |