Publicaciones 2011-2020

@article{RN387,
title = {Telomerase Inhibition by a New Synthetic Derivative of the Aporphine Alkaloid Boldine},
author = { S.K. Noureini and M. Kheirabadi and F. Masoumi and F. Khosrogerdi and Y. Zarei and C. Suarez-Rozas and J. Salas-Norambuena and B.K. Cassels},
url = {/brokenurl#<Go to ISI>://WOS:000434978700318},
doi = {10.3390/ijms19041239},
issn = {1422-0067},
year = {2018},
date = {2018-01-01},
journal = {International Journal of Molecular Sciences},
volume = {19},
number = {4},
abstract = {Telomerase, the enzyme responsible for cell immortality, is an important target in anti-cancer drug discovery. Boldine, an abundant aporphine alkaloid of Peumus boldus, is known to inhibit telomerase at non-toxic concentrations. Cytotoxicity of N-benzylsecoboldine hydrochloride (BSB), a synthetic derivative of boldine, was determined using the MTT method in MCF7 and MDA-MB231 cells. Aliquots of cell lysates were incubated with various concentrations of BSB in qTRAP (quantitative telomere repeat amplification protocol)-ligand experiments before substrate elongation by telomerase or amplification by hot-start Taq polymerase. The crystal structure of TERT, the catalytic subunit of telomerase from Tribolium castaneum, was used for docking and molecular dynamics analysis. The qTRAP-ligand data gave an IC50 value of about 0.17 +/- 0.1 mu M for BSB, roughly 400 times stronger than boldine, while the LD50 in the cytotoxicity assays were 12.5 and 21.88 mu M, respectively, in cells treated for 48 h. Although both compounds interacted well with the active site, MD analysis suggests a second binding site with which BSB interacts via two hydrogen bonds, much more strongly than boldine. Theoretical analyses also evaluated the IC50 for BSB as submicromolar. BSB, with greater hydrophobicity and flexibility than boldine, represents a promising structure to inhibit telomerase at non-toxic concentrations.},
keywords = {apoptosis, assay binding boldine, cells, derivative, domain, inhibition, n-benzylsecoboldine, site, stress, telomerase},
pubstate = {published},
tppubtype = {article}
}

@article{RN368,
title = {A Review of Raman, Surface-Enhanced Raman Scattering (Sers) and Related Spectroscopic Techniques Applied to Biomolecules in Biomaterials},
author = { F. Celis and M. Garcia and G. Diaz-Fleming and M. Campos-Vallette},
url = {/brokenurl#<Go to ISI>://WOS:000417443500015},
issn = {0717-9707},
year = {2017},
date = {2017-01-01},
journal = {Journal of the Chilean Chemical Society},
volume = {62},
number = {3},
pages = {3627-3632},
abstract = {The development of new biomaterials has gained increasing attention in the last decade. One of the most important aspects in the development of these new materials is to understand the chemical cues presents in the native niche. Among all the techniques currently available for measuring those interactions, Raman spectroscopy offers a unique and non-invasive tool for exploring the behavior of the components within a given biomaterial and their surrounding microenvironment. This technique exploits the unique molecular vibrational fingerprints for pinpointing those interactions. The vibrational response can be improved to the single molecule level, in the presence of metal nanoparticles (NPs) with plasmonic properties (silver, gold and copper) in the so-called SurfaceEnhanced Raman Scattering (SERS), which can be used for in-situ measurements. Another technique recently developed is the Shell-Isolated Nanoparticle-Enhanced Raman Spectroscopy (SHINERS), which overcomes signal contamination from chemical interactions between biomolecules and the metal surface; it does this by coating the metal surface with an inert layer of alumina or silica. In the present contribution, the role and the applications of Raman, SERS and related spectroscopic techniques in the study of biomolecules in biomaterials are reviewed and discussed.},
keywords = {adsorption, biomolecules, bone, calcium-phosphate, cells, fragments, gold living nanoparticles, peptide raman, sers, silver terminal ultraviolet},
pubstate = {published},
tppubtype = {article}
}

@article{RN307,
title = {Improvement of Photovoltaic Performance by Substituent Effect of Donor and Acceptor Structure of Tpa-Based Dye-Sensitized Solar Cells},
author = { N. Inostroza and F. Mendizabal and R. Arratia-P\'{e}rez and C. Orellana and C. Linares-Flores},
url = {/brokenurl#<Go to ISI>://WOS:000369312100025},
doi = {10.1007/s00894-015-2893-9},
issn = {1610-2940},
year = {2016},
date = {2016-01-01},
journal = {Journal of Molecular Modeling},
volume = {22},
number = {1},
abstract = {We report a computational study of a series of organic dyes built with triphenylamine (TPA) as an electron donor group. We designed a set of six dyes called (TPA-n, where n=0-5). In order to enhance the electron-injection process, the electron-donor effect of some specific substituent was studied. Thus, we gave insights into the rational design of organic TPA-based chromophores for use in dye-sensitized solar cells (DSSCs). In addition, we report the HOMO, LUMO, the calculated excited state oxidized potential E-dye*(eV) and the free energy change for electron-injection Delta G(inject)(eV), and the UV-visible absorption bands for TPA-n dyes by a time-dependent density functional theory (TDDFT) procedure at the B3LYP and CAM-B3LYP levels with solvent effect. The results demonstrate that the introduction of the electron-acceptor groups produces an intramolecular charge transfer showing a shift of the absorption wavelengths of TPA-n under studies.},
keywords = {approximation, cells, density design, dye-sensitized electron-transfer, electronic energy-levels, free functional molecular organic organic-dyes, porphyrins, semiconductor, solar spectra, surface theory, tio2},
pubstate = {published},
tppubtype = {article}
}

@article{RN326,
title = {Determinants of Anti-Cancer Effect of Mitochondrial Electron Transport Chain Inhibitors: Bioenergetic Profile and Metabolic Flexibility of Cancer Cells},
author = { F.A. Urra and B. Weiss-Lopez and R. Araya-Maturana},
url = {/brokenurl#<Go to ISI>://WOS:000390650400011},
doi = {10.2174/1381612822666160719122626},
issn = {1381-6128},
year = {2016},
date = {2016-01-01},
journal = {Current Pharmaceutical Design},
volume = {22},
number = {39},
pages = {5998-6008},
abstract = {Recent evidence highlights that energy requirements of cancer cells vary greatly from normal cells and they exhibit different metabolic phenotypes with variable participation of both glycolysis and oxidative phosphorylation (OXPHOS). Interestingly, mitochondrial electron transport chain (ETC) has been identified as an essential component in bioenergetics, biosynthesis and redox control during proliferation and metastasis of cancer cells. This dependence converts ETC of cancer cells in a promising target to design small molecules with anti-cancer actions. Several small molecules have been described as ETC inhibitors with different consequences on mitochondrial bioenergetics, viability and proliferation of cancer cells, when the substrate availability is controlled to favor either the glycolytic or OXPHOS pathway. These ETC inhibitors can be grouped as 1) inhibitors of a respiratory complex (e.g. rotenoids, vanilloids, alkaloids, biguanides and polyphenols), 2) inhibitors of several respiratory complexes (e.g. capsaicin, ME-344 and epigallocatechin-3 gallate) and 3) inhibitors of ETC activity (e.g. elesclomol and VLX600). Although pharmacological ETC inhibition may produce cell death and a decrease of proliferation of cancer cells, factors such as degree of inhibition of ETC activity by small molecules, bioenergetic profile and metabolic flexibility of different cancer types or subpopulations of cells in a particular cancer type, can affect the impact of the anti-cancer actions. Particularly interesting are the adaptive mechanisms induced by ETC inhibition, such as induction of glutamine-dependent reductive carboxylation, which may offer a strategy to sensitize cancer cells to inhibitors of glutamine metabolism.},
keywords = {alpha-ketoglutarate, anti-cancer cancer carboxylation, cells, complex-i, complexes, glutamine glutamine-metabolism, impairment, metabolic metabolism, mitochondrial nadh-ubiquinone ovarian-cancer, oxidative oxidative-phosphorylation, oxidoreductase, pancreatic-cancer, phosphorylation, reductive remodeling, respiratory respiratory-chain, slow-cycling solid tumor-cells, tumors},
pubstate = {published},
tppubtype = {article}
}

@article{RN125,
title = {Design, Synthesis and Cellular Dynamics Studies in Membranes of a New Coumarin-Based "Turn-Off" Fluorescent Probe Selective for Fe2+},
author = { O. Garcia-Beltran and N. Mena and O. Yanez and J. Caballero and V. Vargas and M. Nunez and B.K. Cassels},
url = {/brokenurl#<Go to ISI>://WOS:000325121800007},
doi = {10.1016/j.ejmech.2013.06.022},
issn = {0223-5234},
year = {2013},
date = {2013-01-01},
journal = {European Journal of Medicinal Chemistry},
volume = {67},
pages = {60-63},
publisher = {2013 Elsevier Masson SAS.},
abstract = {A new coumarin-based 'turn-off' fluorescent probe, 7-(diethylamino)-N-(1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl)-2-oxo-2H-chromene-3-carboxamide (AGD) was synthesized. This compound is highly selective for ferrous ions (Fe2+) and can reversibly detect them in aqueous medium. The probe localizes to the cell membrane in living cells, where it can detect changes in Fe2+ concentration. Molecular dynamics (MD) simulations indicate that AGD interacts with the lipid bilayer at the level of the glycerol moieties.},
keywords = {cells, chelatable chelators, chemosensor, deficiency, dynamics, fe2+ fe3+ fluorescent ion, iron, labile molecular probes, sensor, turn-off},
pubstate = {published},
tppubtype = {article}
}