2017 |
Simirgiotis, M J; Quispe, C; Mocan, A; Villatoro, J M; Areche, C; Borquez, J; Sepulveda, B; Echiburu-Chau, C Uhplc High Resolution Orbitrap Metabolomic Fingerprinting of the Unique Species Ophryosporus Triangularis Meyen from the Atacama Desert, Northern Chile Artículo de revista Revista Brasileira De Farmacognosia-Brazilian Journal of Pharmacognosy, 27 (2), pp. 179-187, 2017, ISSN: 0102-695x. Resumen | Enlaces | BibTeX | Etiquetas: analysis, antioxidant benzofurans capacities, chilean constituents, dad-ms, driven esi-tof-ms, essential flavonoids, high liquid-chromatography, mass mass-spectrometry, oils, ophryosporus, orbitrap paposo performance phenolic-compounds, plants, resolution spectrometry, uhplc, valley @article{RN336, title = {Uhplc High Resolution Orbitrap Metabolomic Fingerprinting of the Unique Species Ophryosporus Triangularis Meyen from the Atacama Desert, Northern Chile}, author = { M.J. Simirgiotis and C. Quispe and A. Mocan and J.M. Villatoro and C. Areche and J. Borquez and B. Sepulveda and C. Echiburu-Chau}, url = {/brokenurl#<Go to ISI>://WOS:000397844800008}, doi = {10.1016/j.bjp.2016.10.002}, issn = {0102-695x}, year = {2017}, date = {2017-01-01}, journal = {Revista Brasileira De Farmacognosia-Brazilian Journal of Pharmacognosy}, volume = {27}, number = {2}, pages = {179-187}, publisher = {2016 Sociedade Brasileira de Farmacognosia. Published by Elsevier Editora Ltda.}, abstract = {High-resolution mass spectrometry is currently used to determine the mass of biologically active compounds in plants and UHPLC-Orbitrap is a relatively new technology that allows fast fingerprinting and metabolomics analysis. In this work, several phenolic compounds including eleven phenolic acids, two fatty acids, two chromones and fourteen flavones were rapidly identified in the methanolic extracts of aerial parts and flowers of the unique Chilean species Ophryosporus triangularis Meyen, Asteraceae, growing in the Atacama Desert by means of ultrahigh resolution liquid chromatography orbitrap MS analysis (UHPLC-PDA-OT-MS) for the first time. The UHPLC-MS fingerprint generated can be used for the authentication of this endemic species. The methanolic extracts of the aerial parts and flowers showedalso antioxidant capacities (65.34 +/- 1.32 and 52.41 +/- 1.87 mu g/ml in the DPPH assay, 184.88 +/- 13.22 and 196.80 +/- 13.28 mu mol TE/g dry weight in the ferric reducing power assay and 56.17 +/- 3.03 and 65.41 +/- 1.96% in the superoxide anion scavenging assay, respectively).}, keywords = {analysis, antioxidant benzofurans capacities, chilean constituents, dad-ms, driven esi-tof-ms, essential flavonoids, high liquid-chromatography, mass mass-spectrometry, oils, ophryosporus, orbitrap paposo performance phenolic-compounds, plants, resolution spectrometry, uhplc, valley}, pubstate = {published}, tppubtype = {article} } High-resolution mass spectrometry is currently used to determine the mass of biologically active compounds in plants and UHPLC-Orbitrap is a relatively new technology that allows fast fingerprinting and metabolomics analysis. In this work, several phenolic compounds including eleven phenolic acids, two fatty acids, two chromones and fourteen flavones were rapidly identified in the methanolic extracts of aerial parts and flowers of the unique Chilean species Ophryosporus triangularis Meyen, Asteraceae, growing in the Atacama Desert by means of ultrahigh resolution liquid chromatography orbitrap MS analysis (UHPLC-PDA-OT-MS) for the first time. The UHPLC-MS fingerprint generated can be used for the authentication of this endemic species. The methanolic extracts of the aerial parts and flowers showedalso antioxidant capacities (65.34 +/- 1.32 and 52.41 +/- 1.87 mu g/ml in the DPPH assay, 184.88 +/- 13.22 and 196.80 +/- 13.28 mu mol TE/g dry weight in the ferric reducing power assay and 56.17 +/- 3.03 and 65.41 +/- 1.96% in the superoxide anion scavenging assay, respectively). |
2016 |
Jara-Bermeo, A; Penailillo, P; San-Martin, A; Malagon, O; Gilardoni, G; Gutierrez, M Chemical Composition and Antibacterial Activity of Essential Oils from Azorella Spinosa (Apiaceae) against Wild Phytopathogenic Bacteria Artículo de revista Journal of the Chilean Chemical Society, 61 (4), pp. 3246-3249, 2016, ISSN: 0717-9707. Resumen | Enlaces | BibTeX | Etiquetas: activity, antimicrobial antimicrobialactivity, antioxidant, azorella compacta, essential extracts, lamiaceae, oils, plant spinosa, thymus-vulgaris, umbelliferae @article{RN284, title = {Chemical Composition and Antibacterial Activity of Essential Oils from Azorella Spinosa (Apiaceae) against Wild Phytopathogenic Bacteria}, author = { A. Jara-Bermeo and P. Penailillo and A. San-Martin and O. Malagon and G. Gilardoni and M. Gutierrez}, url = {/brokenurl#<Go to ISI>://WOS:000393079900019}, doi = {10.4067/S0717-97072016000400019}, issn = {0717-9707}, year = {2016}, date = {2016-01-01}, journal = {Journal of the Chilean Chemical Society}, volume = {61}, number = {4}, pages = {3246-3249}, abstract = {The aim of the present investigation was to appraise variation in the chemical composition and antimicrobial activity against phytopatogenic bacterial of essential oils from Azorella spinosa. Three samples of A. spinosa were collected in different date in the same area. The essential oils were obtained for hydrodestillation using a Clevenger-type apparatus. The chemical composition of essential oils were analysed using Gas chromatography-mass spectrometry showed variation in the yield and composition, the main detected compounds were monoterpenes such as limonene (10.66-12.30%), tricyclene (10.81-16.99%) and sabinene (9.10-11.49%), the most abundant sesquiterpene was gamma-gurjenene (5.48-8.47%). The biological activity of essential oils from A. spinosa against wild phytopatogenic microorganisms showed selectivity over Pseudomonas syringae. This is the first report of the essential oil composition of A. spinosa, and confirms that natural products obtained from aromatic plants represent an important source of bioactive molecules that could be used as new alternatives for the treatment of phytopatogenic bacteria infections.}, keywords = {activity, antimicrobial antimicrobialactivity, antioxidant, azorella compacta, essential extracts, lamiaceae, oils, plant spinosa, thymus-vulgaris, umbelliferae}, pubstate = {published}, tppubtype = {article} } The aim of the present investigation was to appraise variation in the chemical composition and antimicrobial activity against phytopatogenic bacterial of essential oils from Azorella spinosa. Three samples of A. spinosa were collected in different date in the same area. The essential oils were obtained for hydrodestillation using a Clevenger-type apparatus. The chemical composition of essential oils were analysed using Gas chromatography-mass spectrometry showed variation in the yield and composition, the main detected compounds were monoterpenes such as limonene (10.66-12.30%), tricyclene (10.81-16.99%) and sabinene (9.10-11.49%), the most abundant sesquiterpene was gamma-gurjenene (5.48-8.47%). The biological activity of essential oils from A. spinosa against wild phytopatogenic microorganisms showed selectivity over Pseudomonas syringae. This is the first report of the essential oil composition of A. spinosa, and confirms that natural products obtained from aromatic plants represent an important source of bioactive molecules that could be used as new alternatives for the treatment of phytopatogenic bacteria infections. |
2017 |
Uhplc High Resolution Orbitrap Metabolomic Fingerprinting of the Unique Species Ophryosporus Triangularis Meyen from the Atacama Desert, Northern Chile Artículo de revista Revista Brasileira De Farmacognosia-Brazilian Journal of Pharmacognosy, 27 (2), pp. 179-187, 2017, ISSN: 0102-695x. |
2016 |
Chemical Composition and Antibacterial Activity of Essential Oils from Azorella Spinosa (Apiaceae) against Wild Phytopathogenic Bacteria Artículo de revista Journal of the Chilean Chemical Society, 61 (4), pp. 3246-3249, 2016, ISSN: 0717-9707. |