2017 |
Simirgiotis, M J; Quispe, C; Mocan, A; Villatoro, J M; Areche, C; Borquez, J; Sepulveda, B; Echiburu-Chau, C Uhplc High Resolution Orbitrap Metabolomic Fingerprinting of the Unique Species Ophryosporus Triangularis Meyen from the Atacama Desert, Northern Chile Artículo de revista Revista Brasileira De Farmacognosia-Brazilian Journal of Pharmacognosy, 27 (2), pp. 179-187, 2017, ISSN: 0102-695x. Resumen | Enlaces | BibTeX | Etiquetas: analysis, antioxidant benzofurans capacities, chilean constituents, dad-ms, driven esi-tof-ms, essential flavonoids, high liquid-chromatography, mass mass-spectrometry, oils, ophryosporus, orbitrap paposo performance phenolic-compounds, plants, resolution spectrometry, uhplc, valley @article{RN336, title = {Uhplc High Resolution Orbitrap Metabolomic Fingerprinting of the Unique Species Ophryosporus Triangularis Meyen from the Atacama Desert, Northern Chile}, author = { M.J. Simirgiotis and C. Quispe and A. Mocan and J.M. Villatoro and C. Areche and J. Borquez and B. Sepulveda and C. Echiburu-Chau}, url = {/brokenurl#<Go to ISI>://WOS:000397844800008}, doi = {10.1016/j.bjp.2016.10.002}, issn = {0102-695x}, year = {2017}, date = {2017-01-01}, journal = {Revista Brasileira De Farmacognosia-Brazilian Journal of Pharmacognosy}, volume = {27}, number = {2}, pages = {179-187}, publisher = {2016 Sociedade Brasileira de Farmacognosia. Published by Elsevier Editora Ltda.}, abstract = {High-resolution mass spectrometry is currently used to determine the mass of biologically active compounds in plants and UHPLC-Orbitrap is a relatively new technology that allows fast fingerprinting and metabolomics analysis. In this work, several phenolic compounds including eleven phenolic acids, two fatty acids, two chromones and fourteen flavones were rapidly identified in the methanolic extracts of aerial parts and flowers of the unique Chilean species Ophryosporus triangularis Meyen, Asteraceae, growing in the Atacama Desert by means of ultrahigh resolution liquid chromatography orbitrap MS analysis (UHPLC-PDA-OT-MS) for the first time. The UHPLC-MS fingerprint generated can be used for the authentication of this endemic species. The methanolic extracts of the aerial parts and flowers showedalso antioxidant capacities (65.34 +/- 1.32 and 52.41 +/- 1.87 mu g/ml in the DPPH assay, 184.88 +/- 13.22 and 196.80 +/- 13.28 mu mol TE/g dry weight in the ferric reducing power assay and 56.17 +/- 3.03 and 65.41 +/- 1.96% in the superoxide anion scavenging assay, respectively).}, keywords = {analysis, antioxidant benzofurans capacities, chilean constituents, dad-ms, driven esi-tof-ms, essential flavonoids, high liquid-chromatography, mass mass-spectrometry, oils, ophryosporus, orbitrap paposo performance phenolic-compounds, plants, resolution spectrometry, uhplc, valley}, pubstate = {published}, tppubtype = {article} } High-resolution mass spectrometry is currently used to determine the mass of biologically active compounds in plants and UHPLC-Orbitrap is a relatively new technology that allows fast fingerprinting and metabolomics analysis. In this work, several phenolic compounds including eleven phenolic acids, two fatty acids, two chromones and fourteen flavones were rapidly identified in the methanolic extracts of aerial parts and flowers of the unique Chilean species Ophryosporus triangularis Meyen, Asteraceae, growing in the Atacama Desert by means of ultrahigh resolution liquid chromatography orbitrap MS analysis (UHPLC-PDA-OT-MS) for the first time. The UHPLC-MS fingerprint generated can be used for the authentication of this endemic species. The methanolic extracts of the aerial parts and flowers showedalso antioxidant capacities (65.34 +/- 1.32 and 52.41 +/- 1.87 mu g/ml in the DPPH assay, 184.88 +/- 13.22 and 196.80 +/- 13.28 mu mol TE/g dry weight in the ferric reducing power assay and 56.17 +/- 3.03 and 65.41 +/- 1.96% in the superoxide anion scavenging assay, respectively). |
2015 |
Simirgiotis, M J; Benites, J; Areche, C; Sepulveda, B Antioxidant Capacities and Analysis of Phenolic Compounds in Three Endemic Nolana Species by Hplc-Pda-Esi-Ms Artículo de revista Molecules, 20 (6), pp. 11490-11507, 2015, ISSN: 1420-3049. Resumen | Enlaces | BibTeX | Etiquetas: antioxidant atacama berries, capacity, chilean constituents, desert, diterpenoids, extracts fruits, hplc-ms, nolana, paposo phenolics, plants, quantification, valley @article{RN237, title = {Antioxidant Capacities and Analysis of Phenolic Compounds in Three Endemic Nolana Species by Hplc-Pda-Esi-Ms}, author = { M.J. Simirgiotis and J. Benites and C. Areche and B. Sepulveda}, url = {/brokenurl#<Go to ISI>://WOS:000357992700121}, doi = {10.3390/molecules200611490}, issn = {1420-3049}, year = {2015}, date = {2015-01-01}, journal = {Molecules}, volume = {20}, number = {6}, pages = {11490-11507}, abstract = {The antioxidant features, polyphenolic composition and chromatographic fingerprints of the aerial parts from three Chilean endemic plants from the Paposo Valley located on the cost of the Atacama Desert were investigated for the first time using high pressure liquid chromatography coupled with photodiode array detector and electrospray ionization mass analysis (HPLC-PDA-ESI-MS) and spectroscopic methods. The phenolic fingerprints obtained for the plants were compared and correlated with the antioxidant capacities measured by the bleaching of the DPPH radical, the ferric reducing antioxidant power (FRAP) and quantification of the total content of phenolics and flavonoids measured by spectroscopic methods. Thirty phenolics were identified for the first time for these species, mostly phenolic acids, flavanones, flavonols and some of their glycoside derivatives, together with three saturated fatty acids (stearic, palmitic and arachidic acids). Nolana ramosissima showed the highest antioxidant activity (26.35 +/- 1.02 g/mL, 116.07 +/- 3.42 M Trolox equivalents/g dry weight and 81.23% +/- 3.77% of inhibition in the DPPH, FRAP and scavenging activity (SA) assays, respectively), followed by N. aplocaryoides (85.19 +/- 1.64 g/mL, 65.87 +/- 2.33 M TE/g DW and 53.27% +/- 3.07%) and N. leptophylla (124.71 +/- 3.01, 44.23 +/- 5.18 M TE/g DW and 38.63% +/- 1.85%).}, keywords = {antioxidant atacama berries, capacity, chilean constituents, desert, diterpenoids, extracts fruits, hplc-ms, nolana, paposo phenolics, plants, quantification, valley}, pubstate = {published}, tppubtype = {article} } The antioxidant features, polyphenolic composition and chromatographic fingerprints of the aerial parts from three Chilean endemic plants from the Paposo Valley located on the cost of the Atacama Desert were investigated for the first time using high pressure liquid chromatography coupled with photodiode array detector and electrospray ionization mass analysis (HPLC-PDA-ESI-MS) and spectroscopic methods. The phenolic fingerprints obtained for the plants were compared and correlated with the antioxidant capacities measured by the bleaching of the DPPH radical, the ferric reducing antioxidant power (FRAP) and quantification of the total content of phenolics and flavonoids measured by spectroscopic methods. Thirty phenolics were identified for the first time for these species, mostly phenolic acids, flavanones, flavonols and some of their glycoside derivatives, together with three saturated fatty acids (stearic, palmitic and arachidic acids). Nolana ramosissima showed the highest antioxidant activity (26.35 +/- 1.02 g/mL, 116.07 +/- 3.42 M Trolox equivalents/g dry weight and 81.23% +/- 3.77% of inhibition in the DPPH, FRAP and scavenging activity (SA) assays, respectively), followed by N. aplocaryoides (85.19 +/- 1.64 g/mL, 65.87 +/- 2.33 M TE/g DW and 53.27% +/- 3.07%) and N. leptophylla (124.71 +/- 3.01, 44.23 +/- 5.18 M TE/g DW and 38.63% +/- 1.85%). |
2017 |
Uhplc High Resolution Orbitrap Metabolomic Fingerprinting of the Unique Species Ophryosporus Triangularis Meyen from the Atacama Desert, Northern Chile Artículo de revista Revista Brasileira De Farmacognosia-Brazilian Journal of Pharmacognosy, 27 (2), pp. 179-187, 2017, ISSN: 0102-695x. |
2015 |
Antioxidant Capacities and Analysis of Phenolic Compounds in Three Endemic Nolana Species by Hplc-Pda-Esi-Ms Artículo de revista Molecules, 20 (6), pp. 11490-11507, 2015, ISSN: 1420-3049. |