Publicaciones 2011-2020

@article{RN287,
title = {Phenolic Compounds in Chilean Mistletoe (Quintral, Tristerix Tetrandus) Analyzed by Uhplc-Q/Orbitrap/Ms/Ms and Its Antioxidant Properties},
author = { M.J. Simirgiotis and C. Quispe and C. Areche and B. Sepulveda},
url = {/brokenurl#<Go to ISI>://WOS:000373802200140},
doi = {10.3390/molecules21030245},
issn = {1420-3049},
year = {2016},
date = {2016-01-01},
journal = {Molecules},
volume = {21},
number = {3},
abstract = {Mass spectrometry has become a method of choice to characterize bioactive compounds in biological samples because of its sensitivity and selectivity. Hybrid ultra-HPLC hyphenated with Orbitrap mass analyzer is an innovative state of the art technology that allows fast and accurate metabolomic analyses. In this work the metabolites of a Chilean mistletoe endemic to the VIII region of Chile were investigated for the first time using UHPLC mass analysis (UHPLC-PDA-HESI-Orbitrap MSn). The anthocyanins, together with the non-pigmented phenolics were fingerprinted and correlated with the antioxidant capacities measured by the bleaching of the DPPH radical, the ferric reducing antioxidant power (FRAP), the superoxide anion scavenging activity assay (SA), and total content of phenolics, flavonoids and anthocyanins measured by spectroscopic methods. Six anthocyanins were identified, and among them, the 3-O-glycosides of delphinidin and cyanidin were the major ones. In addition, several phenolic acids (including feruloylquinic acid, feruloyl glucose, chlorogenic acid) and several flavonols (luteolin, quercetin, apigenin, isorhamnetin and glycoside derivatives) were also identified. The mistletoe leaves showed the highest antioxidant activity as measured by the DPPH radical bleaching, ferric reducing antioxidant power and superoxide anion scavenging activity tests (13.38 +/- 0.47 mu g/mL, 125.32 +/- 5.96 mu molTE/g DW and 84.06 +/- 4.59 at 100 mu g/mL, respectively).},
keywords = {anthocyanins, antioxidants, bioactive chile, components, del esi-ms, extracts flavonoids, fruits, hplc-dad, hplc-ms, l., liquid-chromatography, mass-spectrometry, medicinal-plants, muerdago, northern phenolic quintral ultra},
pubstate = {published},
tppubtype = {article}
}

@article{RN237,
title = {Antioxidant Capacities and Analysis of Phenolic Compounds in Three Endemic Nolana Species by Hplc-Pda-Esi-Ms},
author = { M.J. Simirgiotis and J. Benites and C. Areche and B. Sepulveda},
url = {/brokenurl#<Go to ISI>://WOS:000357992700121},
doi = {10.3390/molecules200611490},
issn = {1420-3049},
year = {2015},
date = {2015-01-01},
journal = {Molecules},
volume = {20},
number = {6},
pages = {11490-11507},
abstract = {The antioxidant features, polyphenolic composition and chromatographic fingerprints of the aerial parts from three Chilean endemic plants from the Paposo Valley located on the cost of the Atacama Desert were investigated for the first time using high pressure liquid chromatography coupled with photodiode array detector and electrospray ionization mass analysis (HPLC-PDA-ESI-MS) and spectroscopic methods. The phenolic fingerprints obtained for the plants were compared and correlated with the antioxidant capacities measured by the bleaching of the DPPH radical, the ferric reducing antioxidant power (FRAP) and quantification of the total content of phenolics and flavonoids measured by spectroscopic methods. Thirty phenolics were identified for the first time for these species, mostly phenolic acids, flavanones, flavonols and some of their glycoside derivatives, together with three saturated fatty acids (stearic, palmitic and arachidic acids). Nolana ramosissima showed the highest antioxidant activity (26.35 +/- 1.02 g/mL, 116.07 +/- 3.42 M Trolox equivalents/g dry weight and 81.23% +/- 3.77% of inhibition in the DPPH, FRAP and scavenging activity (SA) assays, respectively), followed by N. aplocaryoides (85.19 +/- 1.64 g/mL, 65.87 +/- 2.33 M TE/g DW and 53.27% +/- 3.07%) and N. leptophylla (124.71 +/- 3.01, 44.23 +/- 5.18 M TE/g DW and 38.63% +/- 1.85%).},
keywords = {antioxidant atacama berries, capacity, chilean constituents, desert, diterpenoids, extracts fruits, hplc-ms, nolana, paposo phenolics, plants, quantification, valley},
pubstate = {published},
tppubtype = {article}
}

@article{RN186,
title = {Anthocyanin Characterization, Total Phenolic Quantification and Antioxidant Features of Some Chilean Edible Berry Extracts},
author = { A. Brito and C. Areche and B. Sepulveda and E.J. Kennelly and M.J. Simirgiotis},
url = {/brokenurl#<Go to ISI>://WOS:000341502600014},
doi = {10.3390/molecules190810936},
issn = {1420-3049},
year = {2014},
date = {2014-01-01},
journal = {Molecules},
volume = {19},
number = {8},
pages = {10936-10955},
abstract = {The anthocyanin composition and HPLC fingerprints of six small berries endemic of the VIII region of Chile were investigated using high resolution mass analysis for the first time (HR-ToF-ESI-MS). The antioxidant features of the six endemic species were compared, including a variety of blueberries which is one of the most commercially significant berry crops in Chile. The anthocyanin fingerprints obtained for the fruits were compared and correlated with the antioxidant features measured by the bleaching of the DPPH radical, the ferric reducing antioxidant power (FRAP), the superoxide anion scavenging activity assay (SA), and total content of phenolics, flavonoids and anthocyanins measured by spectroscopic methods. Thirty one anthocyanins were identified, and the major ones were quantified by HPLC-DAD, mostly branched 3-O-glycosides of delphinidin, cyanidin, petunidin, peonidin and malvidin. Three phenolic acids (feruloylquinic acid, chlorogenic acid, and neochlorogenic acid) and five flavonols (hyperoside, isoquercitrin, quercetin, rutin, myricetin and isorhamnetin) were also identified. Calafate fruits showed the highest antioxidant activity (2.33 +/- 0.21 mu g/mL in the DPPH assay), followed by blueberry (3.32 +/- 0.18 mu g/mL), and arrayan (5.88 +/- 0.21), respectively.},
keywords = {activity, antioxidants, arrayan, berberis-microphylla berries, blueberry, calafate, capacity, chequen, chile, cultivars endemic fruits, hplc-ms, inhibitory liquid-chromatography, luma, mass-spectrometry, meli, murtilla, myrtaceae, oxidase poliphenolics, polyphenols, quantitation, southern vegetables},
pubstate = {published},
tppubtype = {article}
}