2014 |
Brito, A; Ramirez, J E; Areche, C; Sepulveda, B; Simirgiotis, M J Hplc-Uv-Ms Profiles of Phenolic Compounds and Antioxidant Activity of Fruits from Three Citrus Species Consumed in Northern Chile Artículo de revista Molecules, 19 (11), pp. 17400-17421, 2014, ISSN: 1420-3049. Resumen | Enlaces | BibTeX | Etiquetas: antioxidants, caenorhabditis-elegans, capacity, chilean dad, damage, de detection, diode-array endemic esi-qtof, fruits, harvest hplc-ms, identification lemon, limon liquid-chromatography, mass-spectrometry, oxidative performance pica pica, poliphenolics, quantitation, species, tandem time @article{RN182, title = {Hplc-Uv-Ms Profiles of Phenolic Compounds and Antioxidant Activity of Fruits from Three Citrus Species Consumed in Northern Chile}, author = { A. Brito and J.E. Ramirez and C. Areche and B. Sepulveda and M.J. Simirgiotis}, url = {/brokenurl#<Go to ISI>://WOS:000345564300019}, doi = {10.3390/molecules191117400}, issn = {1420-3049}, year = {2014}, date = {2014-01-01}, journal = {Molecules}, volume = {19}, number = {11}, pages = {17400-17421}, abstract = {Peels and edible pulp from three species of citrus including Citrus aurantifolia (varieties pica and sutil) and Citrus x lemon var. Genova widely cultivated and consumed in Northern Chile (I and II region) were analyzed for phenolic compounds and antioxidant activity for the first time. A high performance electrospray ionization mass spectrometry (HPLC-UV-ESI-MS) method was developed for the rapid identification of phenolics in extracts from peels and juices of all species. Several flavonoids including one kaempferol-O-hexoside (peak 16) and one hesperidin derivative (peak 22) three quercetin derivatives (peaks 4, 19 and 36), five isorhamnetin derivatives (peaks 5, 23, 24, 26 and 29) four luteolin derivatives (peaks 14, 25, 27 and 40), seven apigenin derivatives (peaks 2, 3, 12, 20, 34, 35 and 39), seven diosmetin derivatives (peaks 7-9, 17, 21, 31 and 37), three chrysoeriol derivatives (peaks 10, 18 and 30), and four eryodictiol derivatives (peaks 6, 13, 15 and 38) were identified in negative and positive mode using full scan mass measurements and MSn fragmentations. Ascorbic acid content was higher in the pulps of the varieties Genova and Sutil (60.13 +/- 1.28 and 56.53 +/- 1.06 mg ascorbic acid per g dry weight, respectively) while total phenolic content was higher in Pica peels followed by Sutil peels (34.59 +/- 0.81 and 25.58 +/- 1.02 mg/g GAE dry weight, respectively). The antioxidant capacity was also higher for Pica peels (10.34 +/- 1.23 mu g/mL in the DPPH assay and 120.63 +/- 2.45 mu M trolox equivalents/g dry weight in the FRAP assay). The antioxidant features together with the high polyphenolic contents can support at least in part, the usage of the peel extracts as nutraceutical supplements, especially to be used as anti-ageing products.}, keywords = {antioxidants, caenorhabditis-elegans, capacity, chilean dad, damage, de detection, diode-array endemic esi-qtof, fruits, harvest hplc-ms, identification lemon, limon liquid-chromatography, mass-spectrometry, oxidative performance pica pica, poliphenolics, quantitation, species, tandem time}, pubstate = {published}, tppubtype = {article} } Peels and edible pulp from three species of citrus including Citrus aurantifolia (varieties pica and sutil) and Citrus x lemon var. Genova widely cultivated and consumed in Northern Chile (I and II region) were analyzed for phenolic compounds and antioxidant activity for the first time. A high performance electrospray ionization mass spectrometry (HPLC-UV-ESI-MS) method was developed for the rapid identification of phenolics in extracts from peels and juices of all species. Several flavonoids including one kaempferol-O-hexoside (peak 16) and one hesperidin derivative (peak 22) three quercetin derivatives (peaks 4, 19 and 36), five isorhamnetin derivatives (peaks 5, 23, 24, 26 and 29) four luteolin derivatives (peaks 14, 25, 27 and 40), seven apigenin derivatives (peaks 2, 3, 12, 20, 34, 35 and 39), seven diosmetin derivatives (peaks 7-9, 17, 21, 31 and 37), three chrysoeriol derivatives (peaks 10, 18 and 30), and four eryodictiol derivatives (peaks 6, 13, 15 and 38) were identified in negative and positive mode using full scan mass measurements and MSn fragmentations. Ascorbic acid content was higher in the pulps of the varieties Genova and Sutil (60.13 +/- 1.28 and 56.53 +/- 1.06 mg ascorbic acid per g dry weight, respectively) while total phenolic content was higher in Pica peels followed by Sutil peels (34.59 +/- 0.81 and 25.58 +/- 1.02 mg/g GAE dry weight, respectively). The antioxidant capacity was also higher for Pica peels (10.34 +/- 1.23 mu g/mL in the DPPH assay and 120.63 +/- 2.45 mu M trolox equivalents/g dry weight in the FRAP assay). The antioxidant features together with the high polyphenolic contents can support at least in part, the usage of the peel extracts as nutraceutical supplements, especially to be used as anti-ageing products. |
Brito, A; Areche, C; Sepulveda, B; Kennelly, E J; Simirgiotis, M J Anthocyanin Characterization, Total Phenolic Quantification and Antioxidant Features of Some Chilean Edible Berry Extracts Artículo de revista Molecules, 19 (8), pp. 10936-10955, 2014, ISSN: 1420-3049. Resumen | Enlaces | BibTeX | Etiquetas: activity, antioxidants, arrayan, berberis-microphylla berries, blueberry, calafate, capacity, chequen, chile, cultivars endemic fruits, hplc-ms, inhibitory liquid-chromatography, luma, mass-spectrometry, meli, murtilla, myrtaceae, oxidase poliphenolics, polyphenols, quantitation, southern vegetables @article{RN186, title = {Anthocyanin Characterization, Total Phenolic Quantification and Antioxidant Features of Some Chilean Edible Berry Extracts}, author = { A. Brito and C. Areche and B. Sepulveda and E.J. Kennelly and M.J. Simirgiotis}, url = {/brokenurl#<Go to ISI>://WOS:000341502600014}, doi = {10.3390/molecules190810936}, issn = {1420-3049}, year = {2014}, date = {2014-01-01}, journal = {Molecules}, volume = {19}, number = {8}, pages = {10936-10955}, abstract = {The anthocyanin composition and HPLC fingerprints of six small berries endemic of the VIII region of Chile were investigated using high resolution mass analysis for the first time (HR-ToF-ESI-MS). The antioxidant features of the six endemic species were compared, including a variety of blueberries which is one of the most commercially significant berry crops in Chile. The anthocyanin fingerprints obtained for the fruits were compared and correlated with the antioxidant features measured by the bleaching of the DPPH radical, the ferric reducing antioxidant power (FRAP), the superoxide anion scavenging activity assay (SA), and total content of phenolics, flavonoids and anthocyanins measured by spectroscopic methods. Thirty one anthocyanins were identified, and the major ones were quantified by HPLC-DAD, mostly branched 3-O-glycosides of delphinidin, cyanidin, petunidin, peonidin and malvidin. Three phenolic acids (feruloylquinic acid, chlorogenic acid, and neochlorogenic acid) and five flavonols (hyperoside, isoquercitrin, quercetin, rutin, myricetin and isorhamnetin) were also identified. Calafate fruits showed the highest antioxidant activity (2.33 +/- 0.21 mu g/mL in the DPPH assay), followed by blueberry (3.32 +/- 0.18 mu g/mL), and arrayan (5.88 +/- 0.21), respectively.}, keywords = {activity, antioxidants, arrayan, berberis-microphylla berries, blueberry, calafate, capacity, chequen, chile, cultivars endemic fruits, hplc-ms, inhibitory liquid-chromatography, luma, mass-spectrometry, meli, murtilla, myrtaceae, oxidase poliphenolics, polyphenols, quantitation, southern vegetables}, pubstate = {published}, tppubtype = {article} } The anthocyanin composition and HPLC fingerprints of six small berries endemic of the VIII region of Chile were investigated using high resolution mass analysis for the first time (HR-ToF-ESI-MS). The antioxidant features of the six endemic species were compared, including a variety of blueberries which is one of the most commercially significant berry crops in Chile. The anthocyanin fingerprints obtained for the fruits were compared and correlated with the antioxidant features measured by the bleaching of the DPPH radical, the ferric reducing antioxidant power (FRAP), the superoxide anion scavenging activity assay (SA), and total content of phenolics, flavonoids and anthocyanins measured by spectroscopic methods. Thirty one anthocyanins were identified, and the major ones were quantified by HPLC-DAD, mostly branched 3-O-glycosides of delphinidin, cyanidin, petunidin, peonidin and malvidin. Three phenolic acids (feruloylquinic acid, chlorogenic acid, and neochlorogenic acid) and five flavonols (hyperoside, isoquercitrin, quercetin, rutin, myricetin and isorhamnetin) were also identified. Calafate fruits showed the highest antioxidant activity (2.33 +/- 0.21 mu g/mL in the DPPH assay), followed by blueberry (3.32 +/- 0.18 mu g/mL), and arrayan (5.88 +/- 0.21), respectively. |
2014 |
Hplc-Uv-Ms Profiles of Phenolic Compounds and Antioxidant Activity of Fruits from Three Citrus Species Consumed in Northern Chile Artículo de revista Molecules, 19 (11), pp. 17400-17421, 2014, ISSN: 1420-3049. |
Anthocyanin Characterization, Total Phenolic Quantification and Antioxidant Features of Some Chilean Edible Berry Extracts Artículo de revista Molecules, 19 (8), pp. 10936-10955, 2014, ISSN: 1420-3049. |