2014 |
Laich, F; Chavez, R; Vaca, I Leucosporidium Escuderoi F.A., Sp Nov., a Basidiomycetous Yeast Associated with an Antarctic Marine Sponge Artículo de revista Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology, 105 (3), pp. 593-601, 2014, ISSN: 0003-6072. Resumen | Enlaces | BibTeX | Etiquetas: Antarctica, ecology gen. leucosporidium, marine new nov., species, sponge, yeast @article{RN187, title = {Leucosporidium Escuderoi F.A., Sp Nov., a Basidiomycetous Yeast Associated with an Antarctic Marine Sponge}, author = { F. Laich and R. Chavez and I. Vaca}, url = {/brokenurl#<Go to ISI>://WOS:000330953700016}, doi = {10.1007/s10482-014-0114-7}, issn = {0003-6072}, year = {2014}, date = {2014-01-01}, journal = {Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology}, volume = {105}, number = {3}, pages = {593-601}, abstract = {A basidiomycetous yeast, strain E2A-C3-II, was isolated from a marine sponge (Hymeniacidon sp.) collected at a depth of 6 m in Fildes Bay, King George Island, Antarctica. The phylogenetic analysis revealed that the yeast isolated is related to Leucosporidium drummii, Leucosporidiella muscorum and to the Leucosporidium scottii group, including Leucosporidiella creatinivora and Leucosporidiella yakutica. The analysis of the nucleotide differences and the genetic distances of the D1/D2 domain of the LSU rDNA gene and 5.8S ITS regions support that strain E2A-C3-II represents a new species. The novel species can be distinguished from L. drummii by its ability to assimilate l-sorbose, l-rhamnose, lactose and ribitol. The maximum temperature for growth was 25 A degrees C. On the basis of morphological, biochemical and physiological characterization, and phylogenetic and nucleotide analysis, a novel basidiomycetous yeast species, Leucosporidium escuderoi f.a., sp. nov., is proposed. The type strain is E2A-C3-IIT (=CBS 12734(T) =CECT 13080(T)). The Mycobank (http://www.mycobank.org) accession number is MB 804654. The nucleotide sequences of D1/D2 domain of the LSU rDNA gene and 5.8S-ITS regions obtained in this work have been deposited in Genbank under the Accession numbers JN181009 and JN197600, respectively.}, keywords = {Antarctica, ecology gen. leucosporidium, marine new nov., species, sponge, yeast}, pubstate = {published}, tppubtype = {article} } A basidiomycetous yeast, strain E2A-C3-II, was isolated from a marine sponge (Hymeniacidon sp.) collected at a depth of 6 m in Fildes Bay, King George Island, Antarctica. The phylogenetic analysis revealed that the yeast isolated is related to Leucosporidium drummii, Leucosporidiella muscorum and to the Leucosporidium scottii group, including Leucosporidiella creatinivora and Leucosporidiella yakutica. The analysis of the nucleotide differences and the genetic distances of the D1/D2 domain of the LSU rDNA gene and 5.8S ITS regions support that strain E2A-C3-II represents a new species. The novel species can be distinguished from L. drummii by its ability to assimilate l-sorbose, l-rhamnose, lactose and ribitol. The maximum temperature for growth was 25 A degrees C. On the basis of morphological, biochemical and physiological characterization, and phylogenetic and nucleotide analysis, a novel basidiomycetous yeast species, Leucosporidium escuderoi f.a., sp. nov., is proposed. The type strain is E2A-C3-IIT (=CBS 12734(T) =CECT 13080(T)). The Mycobank (http://www.mycobank.org) accession number is MB 804654. The nucleotide sequences of D1/D2 domain of the LSU rDNA gene and 5.8S-ITS regions obtained in this work have been deposited in Genbank under the Accession numbers JN181009 and JN197600, respectively. |
Brito, A; Ramirez, J E; Areche, C; Sepulveda, B; Simirgiotis, M J Hplc-Uv-Ms Profiles of Phenolic Compounds and Antioxidant Activity of Fruits from Three Citrus Species Consumed in Northern Chile Artículo de revista Molecules, 19 (11), pp. 17400-17421, 2014, ISSN: 1420-3049. Resumen | Enlaces | BibTeX | Etiquetas: antioxidants, caenorhabditis-elegans, capacity, chilean dad, damage, de detection, diode-array endemic esi-qtof, fruits, harvest hplc-ms, identification lemon, limon liquid-chromatography, mass-spectrometry, oxidative performance pica pica, poliphenolics, quantitation, species, tandem time @article{RN182, title = {Hplc-Uv-Ms Profiles of Phenolic Compounds and Antioxidant Activity of Fruits from Three Citrus Species Consumed in Northern Chile}, author = { A. Brito and J.E. Ramirez and C. Areche and B. Sepulveda and M.J. Simirgiotis}, url = {/brokenurl#<Go to ISI>://WOS:000345564300019}, doi = {10.3390/molecules191117400}, issn = {1420-3049}, year = {2014}, date = {2014-01-01}, journal = {Molecules}, volume = {19}, number = {11}, pages = {17400-17421}, abstract = {Peels and edible pulp from three species of citrus including Citrus aurantifolia (varieties pica and sutil) and Citrus x lemon var. Genova widely cultivated and consumed in Northern Chile (I and II region) were analyzed for phenolic compounds and antioxidant activity for the first time. A high performance electrospray ionization mass spectrometry (HPLC-UV-ESI-MS) method was developed for the rapid identification of phenolics in extracts from peels and juices of all species. Several flavonoids including one kaempferol-O-hexoside (peak 16) and one hesperidin derivative (peak 22) three quercetin derivatives (peaks 4, 19 and 36), five isorhamnetin derivatives (peaks 5, 23, 24, 26 and 29) four luteolin derivatives (peaks 14, 25, 27 and 40), seven apigenin derivatives (peaks 2, 3, 12, 20, 34, 35 and 39), seven diosmetin derivatives (peaks 7-9, 17, 21, 31 and 37), three chrysoeriol derivatives (peaks 10, 18 and 30), and four eryodictiol derivatives (peaks 6, 13, 15 and 38) were identified in negative and positive mode using full scan mass measurements and MSn fragmentations. Ascorbic acid content was higher in the pulps of the varieties Genova and Sutil (60.13 +/- 1.28 and 56.53 +/- 1.06 mg ascorbic acid per g dry weight, respectively) while total phenolic content was higher in Pica peels followed by Sutil peels (34.59 +/- 0.81 and 25.58 +/- 1.02 mg/g GAE dry weight, respectively). The antioxidant capacity was also higher for Pica peels (10.34 +/- 1.23 mu g/mL in the DPPH assay and 120.63 +/- 2.45 mu M trolox equivalents/g dry weight in the FRAP assay). The antioxidant features together with the high polyphenolic contents can support at least in part, the usage of the peel extracts as nutraceutical supplements, especially to be used as anti-ageing products.}, keywords = {antioxidants, caenorhabditis-elegans, capacity, chilean dad, damage, de detection, diode-array endemic esi-qtof, fruits, harvest hplc-ms, identification lemon, limon liquid-chromatography, mass-spectrometry, oxidative performance pica pica, poliphenolics, quantitation, species, tandem time}, pubstate = {published}, tppubtype = {article} } Peels and edible pulp from three species of citrus including Citrus aurantifolia (varieties pica and sutil) and Citrus x lemon var. Genova widely cultivated and consumed in Northern Chile (I and II region) were analyzed for phenolic compounds and antioxidant activity for the first time. A high performance electrospray ionization mass spectrometry (HPLC-UV-ESI-MS) method was developed for the rapid identification of phenolics in extracts from peels and juices of all species. Several flavonoids including one kaempferol-O-hexoside (peak 16) and one hesperidin derivative (peak 22) three quercetin derivatives (peaks 4, 19 and 36), five isorhamnetin derivatives (peaks 5, 23, 24, 26 and 29) four luteolin derivatives (peaks 14, 25, 27 and 40), seven apigenin derivatives (peaks 2, 3, 12, 20, 34, 35 and 39), seven diosmetin derivatives (peaks 7-9, 17, 21, 31 and 37), three chrysoeriol derivatives (peaks 10, 18 and 30), and four eryodictiol derivatives (peaks 6, 13, 15 and 38) were identified in negative and positive mode using full scan mass measurements and MSn fragmentations. Ascorbic acid content was higher in the pulps of the varieties Genova and Sutil (60.13 +/- 1.28 and 56.53 +/- 1.06 mg ascorbic acid per g dry weight, respectively) while total phenolic content was higher in Pica peels followed by Sutil peels (34.59 +/- 0.81 and 25.58 +/- 1.02 mg/g GAE dry weight, respectively). The antioxidant capacity was also higher for Pica peels (10.34 +/- 1.23 mu g/mL in the DPPH assay and 120.63 +/- 2.45 mu M trolox equivalents/g dry weight in the FRAP assay). The antioxidant features together with the high polyphenolic contents can support at least in part, the usage of the peel extracts as nutraceutical supplements, especially to be used as anti-ageing products. |
2014 |
Leucosporidium Escuderoi F.A., Sp Nov., a Basidiomycetous Yeast Associated with an Antarctic Marine Sponge Artículo de revista Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology, 105 (3), pp. 593-601, 2014, ISSN: 0003-6072. |
Hplc-Uv-Ms Profiles of Phenolic Compounds and Antioxidant Activity of Fruits from Three Citrus Species Consumed in Northern Chile Artículo de revista Molecules, 19 (11), pp. 17400-17421, 2014, ISSN: 1420-3049. |