Publicaciones 2011-2020

@article{RN187,
title = {Leucosporidium Escuderoi F.A., Sp Nov., a Basidiomycetous Yeast Associated with an Antarctic Marine Sponge},
author = { F. Laich and R. Chavez and I. Vaca},
url = {/brokenurl#<Go to ISI>://WOS:000330953700016},
doi = {10.1007/s10482-014-0114-7},
issn = {0003-6072},
year = {2014},
date = {2014-01-01},
journal = {Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology},
volume = {105},
number = {3},
pages = {593-601},
abstract = {A basidiomycetous yeast, strain E2A-C3-II, was isolated from a marine sponge (Hymeniacidon sp.) collected at a depth of 6 m in Fildes Bay, King George Island, Antarctica. The phylogenetic analysis revealed that the yeast isolated is related to Leucosporidium drummii, Leucosporidiella muscorum and to the Leucosporidium scottii group, including Leucosporidiella creatinivora and Leucosporidiella yakutica. The analysis of the nucleotide differences and the genetic distances of the D1/D2 domain of the LSU rDNA gene and 5.8S ITS regions support that strain E2A-C3-II represents a new species. The novel species can be distinguished from L. drummii by its ability to assimilate l-sorbose, l-rhamnose, lactose and ribitol. The maximum temperature for growth was 25 A degrees C. On the basis of morphological, biochemical and physiological characterization, and phylogenetic and nucleotide analysis, a novel basidiomycetous yeast species, Leucosporidium escuderoi f.a., sp. nov., is proposed. The type strain is E2A-C3-IIT (=CBS 12734(T) =CECT 13080(T)). The Mycobank (http://www.mycobank.org) accession number is MB 804654. The nucleotide sequences of D1/D2 domain of the LSU rDNA gene and 5.8S-ITS regions obtained in this work have been deposited in Genbank under the Accession numbers JN181009 and JN197600, respectively.},
keywords = {Antarctica, ecology gen. leucosporidium, marine new nov., species, sponge, yeast},
pubstate = {published},
tppubtype = {article}
}

@article{RN37c,
title = {Production of a Heterologous Recombinant Protein Using Fragments of the Glyceraldehyde-3-Phosphate Dehydrogenase Promoter from Penicillium Camemberti},
author = { Y. Espinosa and J. Trebotich and F. Sepulveda and J. Cadena and M.J. Vargas-Straube and I. Vaca and P. Bull and G. Levican and R. Chavez},
url = {/brokenurl#<Go to ISI>://WOS:000297594400033},
doi = {10.1007/s11274-011-0782-7},
issn = {0959-3993},
year = {2011},
date = {2011-01-01},
journal = {World Journal of Microbiology & Biotechnology},
volume = {27},
number = {12},
pages = {3019-3023},
abstract = {The biotechnological applications of cheese-ripening fungi have been limited by a lack of genetics tools, in particular the identification and characterization of suitable promoters for protein expression. In this study, the suitability of the glyceraldehyde-3-phosphate dehydrogenase (gpdP) promoter from Penicillium camemberti to drive the production of a recombinant protein was evaluated. The gpdP gene and its promoter were isolated using PCR and Genome Walker. The promoter of gpdP has two regions with high identity to the regulatory elements gpd-box and ct-box previously described in Aspergillus nidulans. Two fragments of the promoter containing the gpd- and ct-box or the ct-box alone were used to drive the in vivo production of recombinant beta-galactosidase using A. nidulans as host. Our results indicate that larger fragment containing gpd-box enhances the production of beta-galactosidase activity levels respect to ct-box alone, and that both boxes are necessary to obtain maximal enzymatic activity production. The smaller fragment (187 nt) containing the ct-box alone was able to trigger up to 27% of beta-galactosidase activity, and to our knowledge this is the smallest fragment from a gpd gene used to produce a recombinant protein. Differences were not observed when glycerol, galactose or glucose were used as carbon sources, suggesting that the promoter activity is carbohydrate-independent. This is the first report in which a Penicillium gpd promoter is used for recombinant protein production. Our results open the way for the future development of a system for recombinant proteins expression in the biotechnologically important cheese-ripening fungus P. camemberti.},
keywords = {aspergillus-nidulans, beta-galactosidase, camemberti, cloning, dehydrogenase elements, expression, functional gene, glyceraldehyde-3-phosphate gpda heterologous nidulans penicillium promoter, protein region sequence-analysis, transformation, yeast},
pubstate = {published},
tppubtype = {article}
}