Publicaciones 2011-2020

@article{RN383,
title = {Heterologous Expression, Purification and Characterization of a Highly Thermolabile Endoxylanase from the Antarctic Fungus Cladosporium Sp},
author = { C. Gil-Dur\'{a}n and M.C. Ravanal and P. Ubilla and I. Vaca and R. Ch\'{a}vez},
url = {http://www.sciencedirect.com/science/article/pii/S187861461830093X},
doi = {10.1016/j.funbio.2018.05.002},
issn = {1878-6146},
year = {2018},
date = {2018-01-01},
journal = {Fungal Biology},
volume = {122},
number = {9},
pages = {875-882},
abstract = {Numerous endoxylanases from mesophilic fungi have been purified and characterized. However, endoxylanases from cold-adapted fungi, especially those from Antarctica, have been less studied. In this work, a cDNA from the Antarctic fungus Cladosporium sp. with similarity to endoxylanases from glycosyl hydrolase family 10, was cloned and expressed in Pichia pastoris. The pure recombinant enzyme (named XynA) showed optimal activity on xylan at 50 °C and pH 6\textendash7. The enzyme releases xylooligosaccharides but not xylose, indicating that XynA is a classical endoxylanase. The enzyme was most active on xylans with high content of arabinose (rye arabinoylan and wheat arabinoxylan) than on xylans with low content of arabinose (oat spelts xylan, birchwood xylan and beechwood xylan). Finally, XynA showed a very low thermostability. After 20\textendash30 min of incubation at 40 °C, the enzyme was completely inactivated, suggesting that XynA would be the most thermolabile endoxylanase described so far in filamentous fungi. This is one of the few reports describing the heterologous expression and characterization of a xylanase from a fungus isolated from Antarctica.},
keywords = {Antarctica, Cold-active Cold-adapted Low Xylan enzyme, fungus, thermostability},
pubstate = {published},
tppubtype = {article}
}

@article{RN187,
title = {Leucosporidium Escuderoi F.A., Sp Nov., a Basidiomycetous Yeast Associated with an Antarctic Marine Sponge},
author = { F. Laich and R. Chavez and I. Vaca},
url = {/brokenurl#<Go to ISI>://WOS:000330953700016},
doi = {10.1007/s10482-014-0114-7},
issn = {0003-6072},
year = {2014},
date = {2014-01-01},
journal = {Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology},
volume = {105},
number = {3},
pages = {593-601},
abstract = {A basidiomycetous yeast, strain E2A-C3-II, was isolated from a marine sponge (Hymeniacidon sp.) collected at a depth of 6 m in Fildes Bay, King George Island, Antarctica. The phylogenetic analysis revealed that the yeast isolated is related to Leucosporidium drummii, Leucosporidiella muscorum and to the Leucosporidium scottii group, including Leucosporidiella creatinivora and Leucosporidiella yakutica. The analysis of the nucleotide differences and the genetic distances of the D1/D2 domain of the LSU rDNA gene and 5.8S ITS regions support that strain E2A-C3-II represents a new species. The novel species can be distinguished from L. drummii by its ability to assimilate l-sorbose, l-rhamnose, lactose and ribitol. The maximum temperature for growth was 25 A degrees C. On the basis of morphological, biochemical and physiological characterization, and phylogenetic and nucleotide analysis, a novel basidiomycetous yeast species, Leucosporidium escuderoi f.a., sp. nov., is proposed. The type strain is E2A-C3-IIT (=CBS 12734(T) =CECT 13080(T)). The Mycobank (http://www.mycobank.org) accession number is MB 804654. The nucleotide sequences of D1/D2 domain of the LSU rDNA gene and 5.8S-ITS regions obtained in this work have been deposited in Genbank under the Accession numbers JN181009 and JN197600, respectively.},
keywords = {Antarctica, ecology gen. leucosporidium, marine new nov., species, sponge, yeast},
pubstate = {published},
tppubtype = {article}
}