Publicaciones 2011-2020

@article{solar2015multifunctional,
title = {Multifunctional polymeric nanoparticles doubly loaded with SPION and ceftiofur retain their physical and biological properties},
author = { P. Solar and G. Gonz\'{a}lez and C. Vilos and N. Herrera and N. Juica and M. Moreno and F. Simon and L. Vel\'{a}squez},
doi = {Mult10.1186/s12951-015-0077-5},
issn = {1477-3155},
year = {2015},
date = {2015-01-01},
journal = {Journal of Nanobiotechnology},
volume = {13},
abstract = {Advances in nanostructure materials are leading to novel strategies for drug delivery and targeting, contrast media for magnetic resonance imaging (MRI), agents for hyperthermia and nanocarriers. Superparamagnetic iron oxide nanoparticles (SPIONs) are useful for all of these applications, and in drug-release systems, SPIONs allow for the localization, direction and concentration of drugs, providing a broad range of therapeutic applications. In this work, we developed and characterized polymeric nanoparticles based on poly (3-hydroxybutyric acid-co-hydroxyvaleric acid) (PHBV) functionalized with SPIONs and/or the antibiotic ceftiofur. These nanoparticles can be used in multiple biomedical applications, and the hybrid SPION-ceftiofur nanoparticles (PHBV/SPION/CEF) can serve as a multifunctional platform for the diagnosis and treatment of cancer and its associated bacterial infections., Results: Morphological examination using transmission electron microscopy (TEM) showed nanoparticles with a spherical shape and a core-shell structure. The particle size was evaluated using dynamic light scattering (DLS), which revealed a diameter of 243.0 +/- 17 nm. The efficiency of encapsulation (45.5 +/- 0.6% w/v) of these polymeric nanoparticles was high, and their components were evaluated using spectroscopy. UV-VIS, FTIR and DSC showed that all of the nanoparticles contained the desired components, and these compounds interacted to form a nanocomposite. Using the agar diffusion method and live/dead bacterial viability assays, we demonstrated that these nanoparticles have antimicrobial properties against Escherichia coli, and they retain their magnetic properties as measured using a vibrating sample magnetometer (VSM). Cytotoxicity was assessed in HepG2 cells using live/dead viability assays and MTS, and these assays showed low cytotoxicity with IC50 > 10 mg/mL nanoparticles., Conclusions: Our results indicate that hybrid and multifunctional PHBV/SPION/CEF nanoparticles are suitable as a superparamagnetic drug delivery system that can guide, concentrate and site-specifically release drugs with antibacterial activity.},
keywords = {ceftiofur, delivery, drug drug-delivery, magnetic micelles, nanoparticles, phbv, polymeric release spion, superparamagnetic},
pubstate = {published},
tppubtype = {article}
}

@article{RN249,
title = {Gold Nanoparticles Interacting with Beta-Cyclodextrin-Phenylethylamine Inclusion Complex: A Ternary System for Photothermal Drug Release},
author = { R. Sierpe and E. Lang and P. Jara and A.R. Guerrero and B. Chornik and M.J. Kogan and N. Yutronic},
url = {/brokenurl#<Go to ISI>://WOS:000358558300010},
doi = {10.1021/acsami.5b00186},
issn = {1944-8244},
year = {2015},
date = {2015-01-01},
journal = {Acs Applied Materials & Interfaces},
volume = {7},
number = {28},
pages = {15177-15188},
abstract = {We report the synthesis of a 1:1 beta-cydodextrin-phenylethylamine (beta CD-PhEA) inclusion complex (IC) and the adhesion of gold nanoparticles (AuNPs) onto micro crystals of this complex, which forms a ternary system. The formation of the IC was confirmed by powder X-ray diffraction and NMR analyses (H-1 and ROESY). The stability constant of the IC (760 M-1) was determined using the phase solubility method. The adhesion of AuNPs was obtained using the magnetron sputtering technique, and the presence of AuNPs was confirmed using UV-vis spectroscopy (surface plasmon resonance effect), which showed. an absorbance at 533 nm. The powder X-ray diffractograms of beta CD-PhEA were similar to those of the crystals decorated with AuNPs. A comparison of the one- and two-dimensional NMR spectra of the IC with and without AuNPs suggests partial displacement of the guest to the outside of the beta CD due to attraction toward AuNPs, a characteristic tropism effect. The size, morphology, and distribution of the AuNPs were analyzed using TEM and SEM. The average size of the AuNPs was 14 nm. Changes in the IR and Raman spectra were attributed to the formation of the complex and to the specific interactions of this group with the AuNPs. Laser irradiation assays show that the ternary system beta CD-PhEA-AuNPs in solution enables the release of the guest.},
keywords = {applications, beta-cyclodextrin, biomedical brain complex, delivery, effect, enhanced gold inclusion laser-ablation, magnetron nanoparticles, permeability, phenylethylamine, photothermal plasmon resonance, retention, silver size, sputtering, surface},
pubstate = {published},
tppubtype = {article}
}

@article{RN156,
title = {Mitochondria: A Promising Target for Anticancer Alkaloids},
author = { F.A. Urra and M. Cordova-Delgado and H. Pessoa-Mahana and O. Ramirez-Rodriguez and B. Weiss-Lopez and J. Ferreira and R. Araya-Maturana},
url = {/brokenurl#<Go to ISI>://WOS:000324801300007},
issn = {1568-0266},
year = {2013},
date = {2013-01-01},
journal = {Current Topics in Medicinal Chemistry},
volume = {13},
number = {17},
pages = {2171-2183},
abstract = {A great number of alkaloids exhibit high potential in cancer research. Some of them are anticancer drugs with well-defined clinical uses, exerting their action on microtubules dynamics or DNA replication and topology. On the other hand, mitochondria have been recognized as an essential organelle in the establishment of tumor characteristics, especially the resistance to cell death, high proliferative capacity and adaptation to unfavorable cellular environment. Interestingly, many alkaloids exert their anticancer activities affecting selectively some functions of the tumor mitochondria by 1) modulating OXPHOS and ADP/ATP transport, 2) increasing ROS levels and mitochondrial potential dissipation by crosstalk between endoplasmic reticulum (ER) and mitochondria, 3) inducing mitochondria-dependent apoptosis and autophagy, 4) inhibiting mitochondrial metabolic pathways and 5) by alteration of the morphology and biogenesis of this organelle. These antecedents show the relevance of developing research about the effects of alkaloids on functions controlled by tumor mitochondria, offering an attractive target for the design of new alkaloid derivatives, considering organelle-specific delivery strategies. This review describes mitochondria as a central component in the anticancer action of a set of alkaloids, in a way to illustrate the importance of this organelle in medicinal chemistry.},
keywords = {alkaloids, apoptosis, cancer capsaicin carbazole caspase-dependent cells, delivery, endoplasmic endoplasmic-reticulum hepatocellular-carcinoma, human induces melanoma-cells microtubule-modulating mitochondria, mouse nucleotide peptide, prostate-cancer reticulum, self-assembling stress, targeting, translocase},
pubstate = {published},
tppubtype = {article}
}