Publicaciones 2011-2020

@article{RN386,
title = {Variation of the Alkaloid Content of Peumus Boldus (Boldo)},
author = { G. Fuentes-Barros and S. Castro-Saavedra and L. Liberona and W. Acevedo-Fuentes and C. Tirapegui and C. Mattar and B.K. Cassels},
url = {/brokenurl#<Go to ISI>://WOS:000437551000027},
doi = {10.1016/j.fitote.2018.02.020},
issn = {0367-326x},
year = {2018},
date = {2018-01-01},
journal = {Fitoterapia},
volume = {127},
pages = {179-185},
abstract = {Eighteen alkaloids were detected in the bark, leaves, wood and roots of Peumus boldus, including traces of secoboldine, N-methylsecoboldine (boldine methine), glaucine and norreticuline, not reported previously as constituents of this species. Using appropriate standards, we quantified thirteen of them by UHPLC-MS/MS. Boldine was dominant in the bark, and laurolitsine in wood and roots. The alkaloid composition of the leaves, determined for 130 individually identified trees, classified by age and sex, was highly variable, where N-methyllaurotetanine, laurotetanine, coclaurine and in some cases isocorydine predominated, but not boldine.},
keywords = {activity, alkaloids, antioxidant boldine, boldus, herbal in-vitro, inhibition, leaves, medicine, peumus peumus-boldus, phenanthrene phytochemistry, plants, products, reticuline traditional uhplc-ms/ms},
pubstate = {published},
tppubtype = {article}
}

@article{RN289,
title = {Fast Detection of Phenolic Compounds in Extracts of Easter Pears (Pyrus Communis) from the Atacama Desert by Ultrahigh-Performance Liquid Chromatography and Mass Spectrometry (Uhplc-Q/Orbitrap/Ms/Ms)},
author = { M.J. Simirgiotis and C. Quispe and J. Borquez and C. Areche and B. Sepulveda},
url = {/brokenurl#<Go to ISI>://WOS:000369486800014},
doi = {10.3390/molecules21010092},
issn = {1420-3049},
year = {2016},
date = {2016-01-01},
journal = {Molecules},
volume = {21},
number = {1},
abstract = {A small Chilean variety of pears growing in the town of Toconao, an oasis located at the northeastern edge of the Salar de Atacama, northern Chile, was studied by means of modern PDA and high resolution mass spectral data (UHPLC-PDA-HESI-orbitrap-MS/MS). In addition, the antioxidant features of the fruits were compared with the varieties Packhman's Triumph and Abate Fetel and correlated with the presence of phenolic compounds. The non-pigmented phenolics were fingerprinted and related to the antioxidant capacities measured by the bleaching of the DPPH radical, the ferric reducing antioxidant power (FRAP), the superoxide anion scavenging activity assay (SA), and total content of phenolics and flavonoids measured by spectroscopic methods. The machine allowed a fast separation of 15 min employing a flow rate of 1 mL per minute and could accurately identify 25 compounds, including several isorhamnetin derivatives and phenolic acids, present in the peel and pulps of this Chilean variety for the first time. The compounds were monitored using a wavelength range of 210-800 nm. The native small Chilean pear showed the highest antioxidant activity measured as the bleaching of the DPPH radical, the ferric reducing antioxidant power and superoxide anion scavenging activity (8.61 +/- 0.65 g/mL, 712.63 +/- 12.12 micromols trolox equivalents (mol/TE)/100 g FW, and 82.89% +/- 2.52% at 100 g/mL, respectively).},
keywords = {activity, antioxidant capacity, chile, chilean de esi-ms, flavonoids, fruits, hplc-dad, ms/ms, northern orbitrap parts pascua, pda, pears, pera phenolics, proanthocyanidins, products, uhplc-ms},
pubstate = {published},
tppubtype = {article}
}

@article{RN259,
title = {Synthesis, Characterization and in Vitro Biological Evaluation of [Ru(Eta(6)-Arene)(N,N)Cl] Pf6 Compounds Using the Natural Products Arenes Methylisoeugenol and Anethole},
author = { R.A. Delgado and A. Gald\'{a}mez and J. Villena and P.G. Reveco and F.A. Thomet},
url = {/brokenurl#<Go to ISI>://WOS:000351637900020},
doi = {10.1016/j.jorganchem.2014.09.005},
issn = {0022-328x},
year = {2015},
date = {2015-01-01},
journal = {Journal of Organometallic Chemistry},
volume = {782},
pages = {131-137},
publisher = {2014 Elsevier B.V.},
abstract = {Five new organometallic Ru(II) compounds (VI-X) with the general formula [Ru(eta(6)-arene)(N,N)CI]PF6, where arene-N,N correspond to methylisoeugenol-bipyridine (VI); anethole-bipyridine (VII); methylisoeugenol-ethylenediamine (VIII); anethole-ethylenediamine (IX) and methylisoeugenol-1,2-diaminobenzene (X), have been synthesized, fully characterized and biologically evaluated in vitro. The reaction conditions based on the reduction of [Ru(1,5-COD)Cl-2](n) in situ with methyleugenol and estragole, which are natural ligands, induced an alkene isomerization on the allylic substituent of coordinated arenes. The Ru(II)-arene bond formation and isomerization of the C=C bond on the allyl substituent was confirmed using 1H NMR spectroscopy; this result was validated for compound VIII by X-ray diffraction. An XRD analysis revealed the presence of both enantiomers of the complex in the single-crystal. Compounds IX and X exhibited a better cytotoxic activity in vitro than carboplatin, which is a commercial drug, against three human tumor cell lines (MCF-7, PC-3 and HT-29).},
keywords = {bond cancer, complexes, cytotoxicity, diastereomers, drugs, isomerization, natural ovarian oxaliplatin, products, single-crystal, tumor-models},
pubstate = {published},
tppubtype = {article}
}

@article{RN241,
title = {Filamentous Fungi from Extreme Environments as a Promising Source of Novel Bioactive Secondary Metabolites},
author = { R. Chavez and F. Fierro and R.O. Garcia-Rico and I. Vaca},
url = {/brokenurl#<Go to ISI>://WOS:000361157000001},
doi = {10.3389/fmicb.2015.00903},
issn = {1664-302x},
year = {2015},
date = {2015-01-01},
journal = {Frontiers in Microbiology},
volume = {6},
abstract = {Natural product search is undergoing resurgence upon the discovery of a huge previously unknown potential for secondary metabolite (SM) production hidden in microbial genomes. This is also the case for filamentous fungi, since their genomes contain a high number of "orphan" SM gene clusters. Recent estimates indicate that only 5% of existing fungal species have been described, thus the potential for the discovery of novel metabolites in fungi is huge. In this context, fungi thriving in harsh environments are of particular interest since they are outstanding producers of unusual chemical structures. At present, there are around 16 genomes from extreme environment-isolated fungi in databases. In a preliminary analysis of three of these genomes we found that several of the predicted SM gene clusters are probably involved in the biosynthesis of compounds not yet described. Genome mining strategies allow the exploitation of the information in genome sequences for the discovery of new natural compounds. The synergy between genome mining strategies and the expected abundance of SMs in fungi from extreme environments is a promising path to discover new natural compounds as a source of medically useful drugs.},
keywords = {aspergillus-nidulans, biosynthetic clusters, discovery, drug environments, expression, extreme filamentous fungi, gene genome heterologous metabolites, metagenome, metagenomics, mining, natural natural-products, polyketide, products, resource, secondary sequence, strategy},
pubstate = {published},
tppubtype = {article}
}

@article{RN114,
title = {Determination of Antibacterial, Antioxidant, Antiplatelet and Inhibition of Cholinesterase Activities from the Methanolic Extracts of Azorella Species (Apiaceae)},
author = { L. Quesada and M. Gutierrez and L. studillo-Saavedra and A. San-Martin and E. Fuentes and I. Palomo and P. Penailillo},
url = {/brokenurl#<Go to ISI>://WOS:000314060700011},
issn = {0717-7917},
year = {2013},
date = {2013-01-01},
journal = {Boletin Latinoamericano Y Del Caribe De Plantas Medicinales Y Aromaticas},
volume = {12},
number = {1},
pages = {99-107},
abstract = {In this study, we investigated the potential antibacterial, antioxidant and anti-platelet activities and the inhibition of cholinesterase from the methanolic extracts obtained from aerial parts of the two species of Azorella: A. spinosa (Constitution, Chile) and A. monantha (Torres del Paine, Enladrillado and Paso Vergara). All extracts showed only moderate inhibitory activity on acetylcholinesterase (AChE), the most active extract with IC50 = 27 mu g/mL was A. spinosa. Inhibition of platelet aggregation induced by ADP presented maximal aggregation to 70 and 57% on extracts of A. spinosa and A. monantha (Paso Vergara), respectively. The most active extract with antioxidant effect was A. spinosa with IC50 of 28.72 mu g/mL. Antibacterial activity of the extract on Escherichia coli, Pseudomonas aeruginosa and Acinetobacter baumannii was not present. The extracts of A. spinosa and A. monantha (Paso Vergara) presented the best results on the activities that were evaluated.},
keywords = {alzheimers-disease, antibacterial, antioxidant, antiplatelet, azorella, cholinesterase, compacta, diterpenoids, phenolics, products, total yareta},
pubstate = {published},
tppubtype = {article}
}

@article{RN20j,
title = {Extraction and Determination of Oxytetracycline Hydrochloride and Oxolinic Acid in Fish Feed by Derivative Spectrophotometry of First Order},
author = { M.I. Toral and S.L. Orellana and C.A. Soto and P. Richter},
url = {/brokenurl#<Go to ISI>://WOS:000296883500006},
doi = {10.1007/s12161-011-9195-3},
issn = {1936-9751},
year = {2011},
date = {2011-01-01},
journal = {Food Analytical Methods},
volume = {4},
number = {4},
pages = {497-504},
abstract = {In this work is proposed the extraction and determination of oxytetracycline (OTC) and oxolinic acid (OA) in fish feed by first-derivative spectrophotometry. The extractions are carried out by parallel modality, where OTC is extracted in the presence of OA in a sample, and in another is extracted OA in the presence of OTC, and the sequential modality, where OTC is extracted first and then followed by OA in a single feed sample. A phosphate buffer, pH 7.0, was selected for OTC extraction and acetonitrile for OA extraction. These solvents were used in both extraction modalities. The extraction percentages obtained by parallel mode are better than those obtained by sequential extraction. In both cases, the limits of extraction were 25 mg kg(-1) for OTC and 10 mg kg(-1) for OA. However, it is proposed to work with the parallel extraction for its efficiency, accuracy, precision, and less time requirement.},
keywords = {animal antibiotics, chlortetracycline, derivative detection, extractions, feed, feeds, flumequine, fluorescence liquid-chromatography, oxolinic oxytetracycline, performance premixes, products, seawater spectrophotometry, tetracycline water},
pubstate = {published},
tppubtype = {article}
}